OsMYB58 Negatively Regulates Plant Growth and Development by Regulating Phosphate Homeostasis

• Published in: International journal of molecular sciences Vol. 25; no. 4; p. 2209
• Main Authors: Baek, Dongwon, Hong, Soyeon, Kim, Hye Jeong, Moon, Sunok, Jung, Ki Hong, Yang, Won Tae, Kim, Doh Hoon
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-02-2024
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; Biosynthesis; DNA binding proteins; Efficiency; Gene Expression Regulation, Plant; Genes; Genetic aspects; Genetic transcription; Growth; Homeostasis; Micronutrients - metabolism; MYB transcription factor; Oryza - genetics; Oryza - metabolism; OsmiR399; OsMYB58; phosphate starvation signaling; Phosphates; Phosphates - metabolism; PHR-miR399-PHO2; Physiology; Plant Development; Plant growth; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - metabolism; Plant tolerance; Plants - metabolism; R&D; Research & development; Rice; Starvation; Transcription factors; Transcription Factors - metabolism; South Korea; phosphate starvation signaling; PHR-miR399-PHO2; MYB transcription factor; OsMYB58; OsmiR399
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25042209

Phosphate (Pi) starvation is a critical factor limiting crop growth, development, and productivity. Rice (
) R2R3-MYB transcription factors function in the transcriptional regulation of plant responses to various abiotic stresses and micronutrient deprivation, but little is known about their roles in Pi starvation signaling and Pi homeostasis. Here, we identified the R2R3-MYB transcription factor gene
, which shares high sequence similarity with
.
expression was induced more strongly by Pi starvation than by other micronutrient deficiencies. Overexpressing
in
and rice inhibited plant growth and development under Pi-deficient conditions. In addition, the overexpression of
in plants exposed to Pi deficiency strongly affected root development, including seminal root, lateral root, and root hair formation. Overexpressing
strongly decreased the expression of the rice microRNAs
and
. By contrast, overexpressing
strongly increased the expression of rice
(
), whose expression is repressed by miR399 during Pi starvation signaling. OsMYB58 functions as a transcriptional repressor of the expression of its target genes, as determined by a transcriptional activity assay. These results demonstrate that OsMYB58 negatively regulates
-dependent Pi starvation signaling by enhancing
expression.