Mitomycin C, 5-fluorouracil, and cyclosporin A prevent epidural fibrosis in an experimental laminectomy model

• Published in: European spine journal Vol. 16; no. 9; pp. 1525 - 1530
• Main Authors: Yildiz, Kartal Hakan, Gezen, Ferruh, Is, Merih, Cukur, Selma, Dosoglu, Murat
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01-09-2007; Springer-Verlag
• Subjects: Animals; Antibiotics, Antineoplastic - therapeutic use; Cell Count; Cyclosporine - therapeutic use; Epidural Space - pathology; Epidural Space - surgery; Fibroblasts - pathology; Fibrosis - etiology; Fibrosis - prevention & control; Fluorouracil - therapeutic use; Immunosuppressive Agents - therapeutic use; Laminectomy - adverse effects; Lumbar Vertebrae - pathology; Lumbar Vertebrae - surgery; Male; Mitomycin - therapeutic use; Models, Animal; Original; Rats; Rats, Wistar
• ISSN: 0940-6719; 1432-0932
• DOI: 10.1007/s00586-007-0344-8

This study examined the preventive effects of the local application of mitomycin C (MMC), 5-fluorouracil (5-FU), and cyclosporine A (CsA) in minimizing spinal epidural fibrosis in a rat laminectomy model. Thirty-two 2-year-old male Wistar albino rats, each weighing 400 +/- 50 g, were divided into four equal groups: sham, MMC, 5-FU, and CsA. Each rat underwent laminectomy at the L5-L6 lumbar level. Cotton pads (4 x 4 mm2) soaked with MMC (0.5 mg/ml), 5-FU (5 ml/mg), or CsA (5 mg/ml) were placed on the exposed dura for 5 min. Thirty days after surgery, the rats were killed and the epidural fibrosis, fibroblast density, inflammatory cell density, and arachnoid fibrosis were quantified. The epidural and arachnoid fibroses were reduced significantly in the treatment groups compared to the sham group. Fibroblast cell density and inflammatory cell density were decreased significantly in the MMC and 5-FU groups, but were similar in the sham and CsA groups. The decreased rate of epidural fibrosis was promising. Further studies in humans are needed to determine the short- and long-term complications of the agents used here.