Preferential, enhanced breast cancer cell migration on biomimetic electrospun nanofiber 'cell highways'

• Published in: BMC cancer Vol. 14; no. 1; p. 825
• Main Authors: Nelson, Mark Tyler, Short, Aaron, Cole, Sara L, Gross, Amy C, Winter, Jessica, Eubank, Tim D, Lannutti, John J
• Format: Journal Article
• Language: English
• Published: England BioMed Central 10-11-2014
• Subjects: Biomimetic Materials - chemical synthesis; Biopsy; Breast cancer; Breast Neoplasms - chemistry; Breast Neoplasms - pathology; Cancer therapies; Cell adhesion & migration; Cell culture; Cell Movement - drug effects; Chemokine CXCL12 - pharmacology; Chemotactic Factors - pharmacology; Collaboration; Collagen; Colleges & universities; Experiments; Extracellular matrix; Extracellular Matrix - ultrastructure; Female; High-Throughput Screening Assays; Humans; MCF-7 Cells; Medical prognosis; Metastasis; Microscopy; Morphology; Mortality; Nanofibers - ultrastructure; Neoplasm Invasiveness; Neoplasm Metastasis; Patients; Receptors, CXCR4 - genetics; RNA, Messenger - analysis; Tumor Microenvironment
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/1471-2407-14-825

Aggressive metastatic breast cancer cells seemingly evade surgical resection and current therapies, leading to colonization in distant organs and tissues and poor patient prognosis. Therefore, high-throughput in vitro tools allowing rapid, accurate, and novel anti-metastatic drug screening are grossly overdue. Conversely, aligned nanofiber constitutes a prominent component of the late-stage breast tumor margin extracellular matrix. This parallel suggests that the use of a synthetic ECM in the form of a nanoscale model could provide a convenient means of testing the migration potentials of cancer cells to achieve a long-term goal of providing clinicians an in vitro platform technology to test the efficacy of novel experimental anti-metastatic compounds.
Electrospinning produces highly aligned, cell-adhesive nanofiber matrices by applying a strong electric field to a polymer-containing solution. The resulting fibrous microstructure and morphology closely resembles in vivo tumor microenvironments suggesting their use in analysis of migratory potentials of metastatic cancer cells. Additionally, a novel interface with a gel-based delivery system creates CXCL12 chemotactic gradients to enhance CXCR4-expressing cell migration.
Cellular dispersions of MCF-10A normal mammary epithelial cells or human breast cancer cells (MCF-7 and MDA-MB-231) seeded on randomly-oriented nanofiber exhibited no significant differences in total or net distance traveled as a result of the underlying topography. Cells traveled ~2-5 fold greater distances on aligned fiber. Highly-sensitive MDA-MB-231 cells displayed an 82% increase in net distance traversed in the presence of a CXCL12 gradient. In contrast, MCF-7 cells exhibited only 31% increase and MCF-10A cells showed no statistical difference versus control or vehicle conditions. MCF-10A cells displayed little sensitivity to CXCL12 gradients, while MCF-7 cells displayed early sensitivity when CXCL12 concentrations were higher. MDA-MB-231 cells displayed low relative expression levels of CXCR4, but high sensitivity resulting in 55-fold increase at late time points due to CXCL12 gradient dissipation.
This model could create clinical impact as an in vitro diagnostic tool for rapid assessment of tumor needle biopsies to confirm metastatic tumors, their invasiveness, and allow high-throughput drug screening providing rapid development of personalized therapies.