Emerge of NDM-1-Producing Multidrug-Resistant Pseudomonas aeruginosa and Co-Harboring of Carbapenemase Genes in South of Iran

• Published in: Iranian journal of public health Vol. 49; no. 5; pp. 959 - 967
• Main Authors: Farajzadeh Sheikh, Ahmad, Shahin, Mojtaba, Shokoohizadeh, Leili, Ghanbari, Fahimeh, Solgi, Hamid, Shahcheraghi, Fereshteh
• Format: Journal Article
• Language: English
• Published: Iran Tehran University of Medical Sciences 01-05-2020
• Subjects: Acids; Annealing; Antibiotic resistance; Antibiotics; Carbapenemase; Double disk synergy test (DDST); Double-disk potentiation tests (DDPT); Enzymes; Epidemics; Ethylenediaminetetraacetic acids; Genes; Infections; Laboratories; Metallo-β-lactamase; Metallography; Methods; Modified hodge test (MHT); Multidrug resistance; Multidrug resistant organisms; New Delhi metallo-β-lactamase (blaNDM-1); Original; Pseudomonas aeruginosa; Public health; β Lactamase; Pseudomonas aeruginosa; Double-disk potentiation tests (DDPT); Double disk synergy test (DDST); New Delhi metallo-β-lactamase (blaNDM-1); Modified hodge test (MHT)
• ISSN: 2251-6085; 2251-6093
• DOI: 10.18502/ijph.v49i5.3214

New Delhi metallo
beta
lactamase
1 (NDM-1) is one of the most important emerging antibiotic resistance. Co-harboring three or four carbapenemases is rare and only a few reports exist in the literature. We described the characteristics of the large epidemic outbreaks and reports co-producing
with the other carbapenemase genes in
isolates.
This present cross-sectional research was conducted on 369
isolates obtained from burn and general hospitals within years 2013 to 2016. Beta-lactamase classes A, B and D genes were identified by PCR method. Modified hodge test (MHT), double-disk potentiation tests (DDPT) and double disk synergy test (DDST) were performed for detection carbapenemase and metallo beta-lactamase (MBL) production of
positive
isolates.
From 236 carbapenem-resistant
(CRPA), 116 isolates have had MBL genes and twenty-nine isolates were found positive for
. In CRPA isolates,
,
and
were identified in 27.5%, 21.1% and 32.2% of isolates respectively, while co-producing
,
,
, co-producing
,
,
and co-producing
,
were determined in 11 (4.6%), 8 (3.4%) and 27 (11.4%) of isolates respectively.
The finding of this co-existence of multiple carbapenemase resistance genes is threating for public health. Dipicolinic acid is a superior MBL inhibitor in DDPT antique than EDTA in DDST method for the detection of MBL-
producing
.