A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures

Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasit...

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Bibliographic Details
Published in:	Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 78; no. 5; p. 622
Main Authors:	Dluzewski, A R, Ling, I T, Rangachari, K, Bates, P A, Wilson, R J
Format:	Journal Article
Language:	English
Published:	England 1984
Subjects:	Animals Culture Media Erythrocytes - parasitology Parasitology - methods Plasmodium falciparum - growth & development Plasmodium falciparum - isolation & purification Povidone Silicon Dioxide
Online Access:	Get more information
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Summary:	Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
ISSN:	0035-9203
DOI:	10.1016/0035-9203(84)90221-9