Expression of the enzymes of nitrate reduction during the anaerobic germination of rice

• Published in: Plant physiology (Bethesda) Vol. 106; no. 4; pp. 1605 - 1608
• Main Authors: Mattana, M. (Istituto Biosintesi Vegetali, Milano, Italy), Coraggio, I, Bertani, A, Reggiani, R
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-12-1994
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; Agronomy. Soil science and plant productions; ANAEROBIOSE; ANAEROBIOSIS; Antibodies; Biological and medical sciences; CODE GENETIQUE; CODIGO GENETICO; Coleoptiles; Economic plant physiology; Embryo development. Germination; Environmental and Stress Physiology; Enzymes; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENETICA; GENETIQUE; GERMINACION; GERMINATION; Germination and dormancy; Growth and development; Messenger RNA; NITRATE REDUCTASE; Nitrates; NITRATO REDUCTASA; NITRITE REDUCTASE; Nitrites; NITRITO REDUCTASA; ORYZA SATIVA; Plant physiology and development; Plants; Rice; Seedlings; SINTESIS DE PROTEINAS; SYNTHESE PROTEIQUE; Monocotyledones; Enzyme; Nitrite reductase; Germination; Biosynthesis; Gene expression; Cereal crop; Oryza sativa; Gramineae; Anaerobe; Nitrate reductase; Angiospermae; Coleoptile; Spermatophyta; Oxidoreductases
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.106.4.1605

During the anaerobic germination of rice (Oryza sativa L.), nitrate is translocated from the caryopsis and assimilated into the coleoptile (R. Reggiani, M. Mattana, N. Aurisano, A. Bertani [1993] Plant Cell Physiol 34: 379-383). Using antibodies against nitrate and nitrite reductases proteins with the expected molecular mass were recognized by western blot analysis in extracts from 8-d-old rice coleoptiles. Both enzymes are de novo synthesized in 6- to 8-d-old seedlings, as shown by immunoprecipitation of radiolabeled proteins from young plants grown in the presence of [35S]methionine. The anaerobic synthesis of both enzymes was enhanced by the addition of 5 mM KNO3. The effect of exogenous nitrate on the expression of the corresponding genes in anaerobic rice coleoptiles was revealed by the analysis of their transcripts. The importance of the expression of these enzymes during the anaerobic development of rice seedlings is discussed