Streptomyces bangladeshensis sp. nov., isolated from soil, which produces bis-(2-ethylhexyl)phthalate

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 5; pp. 1973 - 1977
• Main Authors: Al-Bari, M. Abdul Alim, Bhuiyan, M. Shah Alam, Flores, Maria Elena, Petrosyan, Pavel, Garcia-Varela, Martin, Islam, M. Anwar Ul
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-09-2005; Society for General Microbiology
• Subjects: Bacteriology; Bangladesh; Biological and medical sciences; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Microbiology; Miscellaneous; Molecular Sequence Data; Phenotype; Phthalic Acids - chemistry; Phthalic Acids - metabolism; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Spores, Bacterial - physiology; Streptomyces; Streptomyces - classification; Streptomyces - genetics; Streptomyces - isolation & purification; Streptomyces - physiology; Streptomyces thermodiastaticus; Streptomyces thermoviolaceus; Streptomycetes; Systematics; Bangladesh; Actinomycetales; Soils; Streptomycetaceae; Taxonomy; Streptomyces; Bacteria; Actinomycetes
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63516-0

1 Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh
2 Laboratory of Molecular Biotechnology, Department of Biotechnology, The University of Tokyo, Yayoi 113-8657, Japan
3 Department of Molecular Biology and Biotechnology, Institute for Biomedical Research, UNAM, A.P. 70228, Mexico, D.F., 04510, Mexico
4 Department of Zoology, Institute of Biology, UNAM, México, D.F., 04510, Mexico
Correspondence María Elena Flores mefc{at}servidor.unam.mx
The taxonomic position of an actinomycete strain isolated from soil from Natore, Bangladesh, was examined by using a polyphasic approach. The strain, designated AAB-4 T , was assigned to the genus Streptomyces on the basis of chemical and morphological criteria. It formed Rectiflexibiles aerial hyphae that carried long chains of rounded spores. The 16S rRNA gene of strain AAB-4 T was sequenced directly and then compared with those of previously studied streptomycetes following the generation of two phylogenetic trees by using maximum-likelihood and neighbour-joining algorithms. This confirmed the assignment of the novel strain to the genus Streptomyces . This strain showed a high level of 16S rRNA gene sequence similarity to Streptomyces thermoviolaceus , Streptomyces thermodiastaticus and Streptomyces longisporus , among others, but could be distinguished from them by phenotypic and physiological traits. This micro-organism produces bis-(2-ethylhexyl)phthalate, an antibacterial and antifungal agent. It is proposed that strain AAB-4 T be classified as a novel species within the genus Streptomyces , as Streptomyces bangladeshensis sp. nov. (type strain, AAB-4 T =LMG 22738 T =NRRL B-24326 T ).
Published online ahead of print on 6 May 2005 as DOI 10.1099/ijs.0.63516-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Streptomyces bangladeshensis strain AAB-4 T (=LMG 22738 T =NRRL B-24326 T ) is AY750056 .
A neighbour-joining dendrogram is available as a supplementary figure in IJSEM Online.