Flower Extracts from Ornamental Plants as Sources of Sunscreen Ingredients: Determination by In Vitro Methods of Photoprotective Efficacy, Antigenotoxicity and Safety

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 17; p. 5525
• Main Authors: Fuentes, Jorge Luis, Pedraza Barrera, Carlos Adolfo, Villamizar Mantilla, Diego Armando, Flórez González, Silvia Juliana, Sierra, Lady Johanna, Ocazionez, Raquel Elvira, Stashenko, Elena E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-08-2022; MDPI
• Subjects: Aging; Analysis; antigenotoxicity; Chlorogenic acid; Cytotoxicity; DNA damage; Fibroblasts; Flowers; Flowers & plants; Genotoxicity; Glucosides; human fibroblasts; Humans; In vitro methods and tests; Ingredients; Kaempferol; Kaempferols; Methods; Mutation; Ornamental plants; photoprotection; Phytochemicals; Plant extracts; Plant Extracts - pharmacology; Plants; Plants (botany); Quercetin; Radiation; Resveratrol; Rosa centifolia; Scientific equipment and supplies industry; Skin cancer; Sunscreen; Sunscreening Agents - pharmacology; Sunscreens (Cosmetics); Toiletries industry; Ultraviolet filters; ultraviolet light; Ultraviolet radiation; Ultraviolet Rays; Colombia; United States; Massachusetts; Germany; genotoxicity; ornamental plants; antigenotoxicity; photoprotection; human fibroblasts; cytotoxicity; ultraviolet light
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27175525

Plants are sources of sunscreen ingredients that prevent cellular mutations involved in skin cancer and aging. This study investigated the sunscreen properties of the extracts from some ornamental plants growing in Colombia. The UV filter capability of the flower extracts obtained from
L.,
(Rudge) Schult, and
Hook. was examined. Photoprotection efficacies were evaluated using in vitro indices such as sun protection factor and critical wavelength. UVB antigenotoxicity estimates measured with the SOS Chromotest were also obtained. Extract cytotoxicity and genotoxicity were studied in human fibroblasts using the trypan blue exclusion and Comet assays, respectively. Major compounds of the promising flower extracts were identified by UHPLC-ESI+-Orbitrap-MS. The studied extracts showed high photoprotection efficacy and antigenotoxicity against UVB radiation, but only the
extract showed broad-spectrum photoprotection at non-cytotoxic concentrations. The
extract appeared to be safer for human fibroblast cells and the
extract was shown to be moderately cytotoxic and genotoxic at the highest assayed concentrations. The
extract was unequivocally cytotoxic and genotoxic. The major constituents of the promising extracts were as follows: chlorogenic acid, ecdysterone 20E, rhamnetin-rutinoside,
-resveratrol-diglucoside,
-resveratrol-diglucoside in
; quercetin, quercetin-glucoside, quercetin-3-rhamnoside, kaempferol, kaempferol-3-glucoside, and kaempferol-rhamnoside in
. The potential of the ornamental plants as sources of sunscreen ingredients was discussed.