Brain Natriuretic Peptide and C-Type Natriuretic Peptide Maintain Porcine Oocyte Meiotic Arrest

• Published in: Journal of cellular physiology Vol. 230; no. 1; pp. 71 - 81
• Main Authors: Zhang, Wenqiang, Yang, Ye, Liu, Wei, Chen, Qian, Wang, Huarong, Wang, Xiao, Zhang, Yanhao, Zhang, Meijia, Xia, Guoliang
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-01-2015; Wiley Subscription Services, Inc
• Subjects: Animals; Cell Cycle Checkpoints; Cells, Cultured; Chorionic Gonadotropin - blood; Chorionic Gonadotropin - pharmacology; Control systems; Female; Granulosa Cells - metabolism; Luteinizing Hormone - metabolism; Meiosis; Natriuretic Peptide, Brain - biosynthesis; Natriuretic Peptide, Brain - physiology; Natriuretic Peptide, C-Type - biosynthesis; Natriuretic Peptide, C-Type - physiology; Oocytes - cytology; Peptides; Pregnancy; Receptors, Atrial Natriuretic Factor - biosynthesis; Swine; Theca Cells - metabolism
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.24682

Recent studies have shown that C‐type natriuretic peptide (CNP) serves as a key control system during mouse oocyte maturation. We used pig models (in vitro and in vivo) to explore the role played by the natriuretic peptide family in porcine oocyte maturation. We reported the expression and location of natriuretic peptide system in different stages of porcine antral follicles. Atrial natriuretic peptide (ANP) and CNP were expressed primarily in granulosa cells, whereas brain natriuretic peptide (BNP) and natriuretic peptide receptor‐B (NPRB) receptor were expressed in granulosa cells (both cumulus and mural granulosa cells) and thecal internal cells, and the natriuretic peptide receptor‐A (NPRA) receptor predominantly in thecal cells. Upon in vitro culture, BNP and CNP maintained meiotic arrest of oocytes associated with cumulus cells. The expression levels of BNP, CNP, and the NPRB receptor increased upon treatment of prepubertal gilts with pregnant mare's serum gonadotropin and decreased upon subsequent human chorionic gonadotropin injection. Such dynamic changes in the expression of natriuretic peptides and their receptor paralleled the proportions of oocytes exhibiting nuclear maturation in vivo. These data indicated that BNP and CNP co‐contributed to maintaining porcine meiotic arrest under physiological condition and lutenizing hormone (LH) relieved this inhibitory effect by decreasing the expression levels of BNP and CNP in vivo. Our present work, combined with previous data, improved the understanding of the oocyte meiotic arrest mechanisms and further revealed that natriuretic peptides serve as oocyte maturation inhibitor (OMI) to inhibit oocyte maturation in mammals. J. Cell. Physiol. 230: 71–81, 2015. © 2014 Wiley Periodicals, Inc.