Identification of MRP-8 (calgranulin A) as a major responsive protein in chronic periodontitis

• Published in: The Journal of pathology Vol. 192; no. 4; pp. 540 - 544
• Main Authors: Lundy, Fionnuala T., Chalk, Rod, Lamey, Philip-John, Shaw, Chris, Linden, Gerard J.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-12-2000; Wiley
• Subjects: Adult; Amino Acid Sequence; Biological and medical sciences; Calcium-Binding Proteins - analysis; Calcium-Binding Proteins - chemistry; Calgranulin A; chemotaxis; Chromatography, High Pressure Liquid; Chronic Disease; chronic periodontitis; Electrophoresis, Polyacrylamide Gel; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; Gingival Crevicular Fluid - chemistry; Humans; inflammation; Male; Medical sciences; Middle Aged; Molecular Sequence Data; MRP-8; Non tumoral diseases; Otorhinolaryngology. Stomatology; Periodontitis - metabolism; Spectrometry, Mass, Electrospray Ionization; Human; Periodontal disease; Chronic; Biochemical analysis; Periodontitis; Stomatology; Pathogenesis; Migration inhibitory factor; HPLC chromatography; Chemotaxis; Mass spectrometry; Gingival fluid
• ISSN: 0022-3417; 1096-9896
• DOI: 10.1002/1096-9896(2000)9999:9999<::AID-PATH740>3.0.CO;2-1

The purpose of the study was to analyse how the protein composition of the inflammatory exudate associated with chronic periodontitis differed from the exudate in periodontal health. Gingival crevicular fluid (GCF) was collected from sites with chronic periodontal inflammation and from non‐diseased sites in healthy control subjects. Microbore HPLC analysis revealed one major difference in GCF protein profiles between healthy controls and periodontitis patients. The protein enhanced in periodontitis patients was identified as migration inhibitory factor‐related protein‐8 (MRP‐8) by a combination of N‐terminal amino acid sequencing, mass spectrometry, and SDS‐PAGE. Together, these data demonstrate, for the first time, the presence of monomeric MRP‐8 in an inflammatory exudate. Whether monomeric MRP‐8 is a unique feature of chronic periodontal inflammation is not yet clear, but the chemotactic properties of this peptide support a functional role for MRP‐8 in periodontal inflammation. Copyright © 2000 John Wiley & Sons, Ltd.