New osmoregulated beta(1-3),beta(1-6) glucosyltransferase(s) in Azospirillum brasilense

• Published in: Journal of Bacteriology Vol. 176; no. 16; pp. 4890 - 4898
• Main Authors: Altabe, S.G, Inon de Iannino, N, Mendoza, D. de, Ugalde, R.A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-08-1994
• Subjects: AZOSPIRILLUM; Azospirillum brasilense - enzymology; Bacteriology; Biological and medical sciences; Cell Compartmentation; Fundamental and applied biological sciences. Psychology; GLICOSILTRANSFERASAS; GLUCANE; GLUCANO; Glucans - biosynthesis; Glucans - chemistry; Glucosyltransferases - metabolism; GLYCOSYLTRANSFERASE; Magnetic Resonance Spectroscopy; Metabolism. Enzymes; Microbiology; OSMOREGULACION; OSMOREGULATION; Periodic Acid - chemistry; PRESION OSMOTICA; PRESSION OSMOTIQUE; SINTESIS DE PROTEINAS; SYNTHESE PROTEIQUE; Water-Electrolyte Balance; Membranous space; Spirillales; Enzyme; Transferases; Glycosyltransferases; Spirillaceae; Azospirillum brasilense; Environmental factor; Biosynthesis; Glucan; Regulation(control); Enzymatic activity; Bacteria; Hexosyltransferases; Osmotic pressure
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.176.16.4890-4898.1994

A linear beta(1-3),beta(1-6) glucan was detected in the periplasm of Azospirillum brasilense cells growing in a medium of low osmotic strength. This glucan was produced in vitro by purified bacterial inner membranes with UDP-glucose as the sugar donor in the presence of Mg2+. Growth in a high-osmotic-strength medium strongly reduced the amount of this glucan accumulated in the periplasmic space, and the inhibition was associated with a reduction in the enzymatic activity of the beta(1-3),beta(1-6) glucosyltransferase(s)