Guided bone regeneration by poly(lactic- co-glycolic acid) grafted hyaluronic acid bi-layer films for periodontal barrier applications

• Published in: Acta biomaterialia Vol. 5; no. 9; pp. 3394 - 3403
• Main Authors: Park, Jung Kyu, Yeom, Junseok, Oh, Eun Ju, Reddy, Mallikarjuna, Kim, Jong Young, Cho, Dong-Woo, Lim, Hyun Pil, Kim, Nam Sook, Park, Sang Won, Shin, Hong-In, Yang, Dong Jun, Park, Kwang Bum, Hahn, Sei Kwang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-11-2009
• Subjects: Adipates - chemistry; Animals; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Bone Regeneration; Carbodiimides - chemistry; Controlled degradation; Guided Tissue Regeneration, Periodontal - methods; Hyaluronic acid; Hyaluronic Acid - chemistry; Hyaluronic Acid - metabolism; Lactic Acid - chemistry; Lactic Acid - metabolism; Materials Testing; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Periodontal barrier membrane; Poly(lactic- co-glycolic acid); Polyglycolic Acid - chemistry; Polyglycolic Acid - metabolism; Rats; Rats, Sprague-Dawley; Skull - cytology; Skull - pathology; Succinimides - chemistry; Poly(lactic- co-glycolic acid); Hyaluronic acid; Bone regeneration; Controlled degradation; Periodontal barrier membrane
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2009.05.019

A novel protocol for the synthesis of biocompatible and degradation controlled poly(lactic-
co-glycolic acid) grafted hyaluronic acid (HA-PLGA) was successfully developed for periodontal barrier applications. HA was chemically modified with adipic acid dihydrazide (ADH) in the mixed solvent of water and ethanol, which resulted in a high degree of HA modification up to 85
mol.%. The stability of HA-ADH to enzymatic degradation by hyaluronidase increased with ADH content in HA-ADH. When the ADH content in HA-ADH was higher than 80
mol.%, HA-ADH became soluble in dimethyl sulfoxide and could be grafted to the activated PLGA with N,N′-dicyclohexyl carbodiimide and N-hydroxysuccinimide. The resulting HA-PLGA was used for the preparation of biphasic periodontal barrier membranes in chloroform. According to in vitro hydrolytic degradation tests in phosphate buffered saline, HA-PLGA/PLGA blend film with a weight ratio of 1/2 degraded relatively slowly compared to PLGA film and HA coated PLGA film. Four different samples of a control, OSSIX
TM membrane, PLGA film, and HA-PLGA/PLGA film were assessed as periodontal barrier membranes for the calvarial critical size bone defects in SD rats. Histological and histomorphometric analyses revealed that HA-PLGA/PLGA film resulted in the most effective bone regeneration compared to other samples with a regenerated bone area of 63.1% covering the bone defect area.