The effects of the broad-specificity lipase inhibitor, tetrahydrolipstatin, on the growth, development and survival of the larvae of Epiphyas postvittana (Walker) (Tortricidae, Lepidoptera)

• Published in: Journal of insect physiology Vol. 57; no. 12; pp. 1643 - 1650
• Main Authors: Markwick, Ngaire P., Poulton, Joanne, McGhie, Tony K., Wohlers, Mark W., Christeller, John T.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-12-2011
• Subjects: Animals; artificial diets; Diet; Epiphyas postvittana; ethanol; Female; Gastric lipase-like; Gastrointestinal Tract - enzymology; Gene Expression; instars; Lactones - pharmacology; Larva - drug effects; Larva - enzymology; Larva - growth & development; larvae; Lepidoptera; Lipase - antagonists & inhibitors; Lipase - genetics; Lipase - metabolism; Lipase inhibitor; Male; midgut; Moths - drug effects; Moths - enzymology; Moths - growth & development; neonates; Orlistat; Pancreatic lipase-like; pupation; RNA, Messenger - metabolism; Tetrahydrolipstatin; Tortricidae; triacylglycerol lipase; Lipase inhibitor; Orlistat; Gastric lipase-like; Pancreatic lipase-like; Gene expression; MLGR; tetrahydrolipstatin
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2011.08.018

Effects of tetrahydrolipstatin (THL) on larval growth to 21
days and percentage pupation of
Epiphyas postvittana larvae.
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Epiphyas postvittana larvae were fed tetrahydrolipstatin (THL) in their diet. ► Survival, growth rate, development, and percentage pupation were reduced. ► Enzyme activity of midgut lipase was reduced in THL-fed larvae. ► Gene expression of gastric lipase genes is up-regulated in THL-fed larvae.
The effects of the lipase inhibitor, tetrahydrolipstatin (THL), on neonate
Epiphyas postvittana (Walker) (Lepidoptera, Tortricidae) larvae were investigated by feeding on control artificial diets (with and without 2% ethanol) and diets containing 2% ethanol and one of three concentrations of THL (0.011%, 0.037% and 0.11%). Small but significant reductions in growth rate, percent pupation and time to pupation were observed for larvae feeding on 2% ethanol control diet compared with standard control diet, but larger reductions in all parameters occurred with increasing THL concentration. Third instar larvae fed 0.011% THL in the diet had 40% of the midgut lipase activity in the relevant control larvae and showed up-regulation of gene expression of the gastric lipase-like family but not the pancreatic lipase-like family of midgut lipases.