UbMES and UbFluor: Novel probes for ring-between-ring (RBR) E3 ubiquitin ligase PARKIN

• Published in: The Journal of biological chemistry Vol. 292; no. 40; pp. 16539 - 16553
• Main Authors: Park, Sungjin, Foote, Peter K., Krist, David T., Rice, Sarah E., Statsyuk, Alexander V.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06-10-2017; American Society for Biochemistry and Molecular Biology
• Subjects: Allosteric Regulation; Animals; enzyme; enzyme mechanism; Enzymology; Fluorescence Polarization - methods; Humans; Molecular Probes - chemistry; Mutation; parkin; Parkinson disease; Polyubiquitin - chemistry; Polyubiquitin - genetics; Polyubiquitin - metabolism; UbFluor; ubiquitin; Ubiquitin-Protein Ligases - chemistry; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitination; enzyme; Parkinson disease; parkin; enzyme mechanism; UbFluor; ubiquitin
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M116.773200

Ring-between-ring (RBR) E3 ligases have been implicated in autoimmune disorders and neurodegenerative diseases. The functions of many RBR E3s are poorly defined, and their regulation is complex, involving post-translational modifications and allosteric regulation with other protein partners. The functional complexity of RBRs, coupled with the complexity of the native ubiquitination reaction that requires ATP and E1 and E2 enzymes, makes it difficult to study these ligases for basic research and therapeutic purposes. To address this challenge, we developed novel chemical probes, ubiquitin C-terminal fluorescein thioesters UbMES and UbFluor, to qualitatively and quantitatively assess the activity of the RBR E3 ligase PARKIN in a simple experimental setup and in real time using fluorescence polarization. First, we confirmed that PARKIN does not require an E2 enzyme for substrate ubiquitination, lysine selection, and polyubiquitin chain formation. Second, we confirmed that UbFluor quantitatively detects naturally occurring activation states of PARKIN caused by Ser65 phosphorylation (pPARKIN) and phosphorylated ubiquitin (pUb). Third, we showed that both pUb and the ubiquitin-accepting substrate contribute to maximal pPARKIN ubiquitin conjugation turnover. pUb enhances the transthiolation step, whereas the substrate clears the pPARKIN∼Ub thioester intermediate. Finally, we established that UbFluor can quantify activation or inhibition of PARKIN by structural mutations. These results demonstrate the feasibility of using UbFluor for quantitative studies of the biochemistry of RBR E3s and for high-throughput screening of small-molecule activators or inhibitors of PARKIN and other RBR E3 ligases.