Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma

• Published in: Evidence-based complementary and alternative medicine Vol. 2011; no. 2011; pp. 1 - 11
• Main Authors: Mishra, Tulika, Khullar, Madhu, Bhatia, Aruna
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01-01-2011; Hindawi Publishing Corporation; Hindawi Limited
• Subjects: Antioxidants; Apoptosis; Ascites; Biotechnology; Cancer; Catalase; Cell cycle; Cell growth; Cell lines; Cell proliferation; DNA fragmentation; Electrophoresis; Ethanol; Extraction plants; Fruit trees; Gel electrophoresis; Glutathione; Hemoglobin; Incubation; Life span; Lipid peroxidation; Mice; Molting; Peroxidation; Plant extracts; Seeds; Superoxide; Superoxide dismutase; Tumor cell lines; Ziziphus mauritiana; United States > US; Patiala India; India
• ISSN: 1741-427X; 1741-4288; 1741-4288; 1741-427X
• DOI: 10.1155/2011/765029

Ziziphus mauritiana (Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.