Fos and Jun oncogenes transactivate chimeric or native promoters containing AP1/GCN4 binding sites in plant cells

• Published in: The Plant cell Vol. 2; no. 7; pp. 651 - 658
• Main Authors: Hilson, P. (Faculte des Sciences Agronomiques de l'Etat, Gembloux, Belgium), Froidmont, D. de, Lejour, C, Hirai, S.I, Jacquemin, J.M, Yaniv, M
• Format: Journal Article
• Language: English
• Published: England American Society of Plant Physiologists 01-07-1990; American Society of Plant Biologists (ASPB)
• Subjects: ACILTRANSFERASA; ACYLTRANSFERASE; ADN; Base Sequence; Binding sites; Binding Sites - genetics; CAULIMOVIRUS; Cells, Cultured; CHIMERE; DNA; DNA-Binding Proteins - genetics; EXPRESION GENICA; EXPRESSION DES GENES; GENE; GENES; Genes, fos; Genes, jun; GENETICA; GENETIQUE; GLICOSIDASAS; GLUTENINAS; GLUTENINE; Glutens - analogs & derivatives; Glutens - genetics; GLYCOSIDASE; Life Sciences; Molecular Sequence Data; NEOPLASMAS; NEOPLASME; NICOTIANA; Nicotiana - genetics; Nicotiana - metabolism; NUCLEOTIDE; NUCLEOTIDOS; Oncogene Proteins v-fos - genetics; Open reading frames; Plant cells; Plants, Toxic; Plasmids; Plasmids - genetics; Promoter regions; Promoter Regions, Genetic - genetics; PROTEINAS; PROTEINE; Proto-Oncogene Proteins c-jun - genetics; Protoplasts; QUIMERA; RATON; Recombinant Fusion Proteins - genetics; SOURIS; Transactivation; Transcriptional Activation - genetics; TRITICUM AESTIVUM; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX; Yeasts
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.2.7.651

The function of mammalian transcription factors of the leucine zipper class was investigated in leaf-derived protoplasts of tobacco. In transient expression experiments, Fos and Jun strongly activated chimeric promoters composed of the TATA box region of the cauliflower mosaic virus 35S transcript preceded by one to five copies of an AP1/GCN4 binding site. Fos and Jun also stimulated a wheat high molecular weight glutenin promoter in which similar binding sites are located more than 500 base pairs from its transcription start site. Both the DNA binding and the transcription activation domains of these proteins were required for proper promoter stimulation by Fos and Jun. Each factor alone was partially active, suggesting that at least the Fos protein can associate with an endogenous plant transcription factor. These observations support the hypothesis that sequences related to AP1/GCN4 binding sites could be cis-acting modules involved in the transcriptional regulation of plant genes