Development and validation of a RP-HPLC method for quantification of isoflavone aglycones in hydrolyzed soy dry extracts

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 836; no. 1; pp. 74 - 78
• Main Authors: da Costa César, Isabela, Braga, Fernão Castro, Soares, Cristina Duarte Vianna, de Aguiar Nunan, Elzíria, Pianetti, Gerson Antônio, Condessa, Felipe Antonacci, Barbosa, Thiago Assis F., Campos, Ligia Maria Moreira
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19-05-2006; Elsevier Science
• Subjects: Acid hydrolysis; Aglycones; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Daidzein; Fundamental and applied biological sciences. Psychology; General pharmacology; Genistein; Glycine max - chemistry; Glycitein; Hydrolysis; Isoflavones; Isoflavones - analysis; Medical sciences; Pharmacology. Drug treatments; Plant Extracts - chemistry; Reproducibility of Results; RP-HPLC; Sensitivity and Specificity; RP-HPLC; Acid hydrolysis; Aglycones; Genistein; Glycitein; Daidzein; Isoflavones; HPLC chromatography; Extract; Flavonoid; Isoflavone derivatives; Polyphenol; Investigation method; Phenols; Development
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.03.030

Isoflavones are widely used as an alternative treatment to hormone replacement therapy and also for prevention of several chronic diseases, including cancers. Genistein, daidzein and glycitein are the most abundant isoflavone aglycones found in soy extracts, where they also occur as glycosides. This paper describes the development and validation of an isocratic reversed-phase HPLC (RP-HPLC) method for the analysis of isoflavone aglycones, released after acid hydrolysis of soy dry extracts, used as pharmaceutical raw material. The quantification was carried out in a C
18 endcapped column, using a mobile phase composed of 0.1% acetic acid and methanol (52:48), at a flow rate of 1.0
ml/min and diode array detection (DAD) at 254
nm. The method showed to be linear (
r
2
>
0.99), precise (R.S.D.
<
2%), accurate (recovery of 98.88% for daidzein and 98.12% for genistein), robust and specific.