An experimental model of chronic rhinosinusitis in rabbits without bacterial inoculation

• Published in: Acta cirurgica brasileira Vol. 29; no. 5; pp. 313 - 319
• Main Authors: Migliavacca, Raphaella de Oliveira, Piltcher, Otávio Bejzman, Kliemann, Lúcia Maria, Cerski, Marcelle Reesink, Meyer, Fabíola, Oppermann, Paula de Oliveira, Machado Filho, Geraldo, Kang, Suzie Hyeona, Costa, Sady Selaimen da
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-05-2014
• Subjects: Animals; Biopsy; Chronic Disease; Disease Models, Animal; Enbucrilate; Male; Maxillary Sinus - pathology; Maxillary Sinus - surgery; Maxillary Sinusitis; Models, Animal; Nasal Cavity - pathology; Nasal Cavity - surgery; Nasal Mucosa - pathology; Rabbits; Reference Values; Reproducibility of Results; Rhinitis - etiology; Rhinitis - pathology; Sinusitis; Sinusitis - etiology; Sinusitis - pathology; Time Factors
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-86502014000500005

Evaluate and compare two different experimental techniques of maxillary sinus ostium occlusion using N-butyl cyanoacrylate in developing chronic histological findings without the inoculation of pathogenic bacteria among rabbits.
In a randomized study, sixteen New Zealand rabbits were assigned for occlusion of the right maxillary sinus through a transmaxillary approach or through the roof of the nasal cavity. The contralateral sinus served as a control. After 12 weeks, the animals were sacrificed for blinded histopathological analysis of the maxillary sinus mucosa.
Histopathological changes consistent with CRS were found in eight (100%) of the maxillary sinuses approached transmaxillary and three of those through the roof of the nasal cavity (37.5%), p 0.008 and 0.250, respectively, comparing with the control side. Chronic mucosal changes were significantly better induced using the transmaxillary approach (p 0.026).
It is possible to induce a model of chronic sinusitis among rabbits with transmaxillary sinus occlusion without bacterial inoculation. This model can be replicated for future cellular studies.