Propionate addition enhances the biodegradation of the xenobiotic herbicide propanil and its metabolite

• Published in: Bioresource technology Vol. 127; pp. 195 - 201
• Main Authors: Oehmen, A., Marques, R., Noronha, J.P., Carvalho, G., Reis, M.A.M.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-01-2013; Elsevier
• Subjects: 3,4-Dichloroaniline (DCA); Acclimation; Agricultural Irrigation; Aniline Compounds - metabolism; Biodegradation; Biodegradation, Environmental - drug effects; Biological and medical sciences; Bioreactors; Biotechnology; Chromatography, High Pressure Liquid; Culture; Degradation; Fundamental and applied biological sciences. Psychology; Herbicides; Herbicides - metabolism; Irrigation; Irrigation waters; Metabolites; mixed culture; oxidative phosphorylation; Oxidative Phosphorylation - drug effects; Propanil; Propanil - metabolism; propionates; Propionates - pharmacology; rivers; Water Pollutants, Chemical - metabolism; Water Purification - methods; Xenobiotics; Portugal, Tejo R; Herbicides; Irrigation waters; Xenobiotics; Propanil; 3,4-Dichloroaniline (DCA); Biodegradation; Irrigation; Metabolite; Pesticides; Propionic acid derivatives; Herbicide; Anilide; Xenobiotic
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.09.120

► Enhanced degradation rates of propanil and DCA achieved with propionate addition. ► Co-metabolism of the xenobiotics with propionate was not a significant mechanism. ► Metabolic model was developed for biodegradation of the herbicide and its metabolite. ► Higher metabolic efficiency of the culture led to the stimulated degradation rates.
This study investigated ways of stimulating the biodegradation rates of the commonly applied herbicide, 3,4-dichloropropionanilide (propanil), and its metabolite, 3,4-dichloroaniline (DCA), as well as the growth rate of propanil- and DCA-degrading organisms in a mixed culture. Propionate, the other metabolite of propanil, stimulated the specific degradation rates of both propanil and DCA after a brief acclimation period. A metabolic model developed to characterise the metabolism of propanil and DCA biodegradation showed that the efficiency of oxidative phosphorylation (i.e. P/O ratio), which measures the metabolic efficiency, increased over time by 6- to 10-fold. This increase was accompanied by a 5- to 10-fold increase in the propanil and DCA biodegradation degradation rates. The biodegradation rates of the culture were unaffected when using an irrigation water matrix (Tejo river, Portugal), highlighting the utility of the culture for bioaugmentation purposes.