Expression of BDNF and TrkB mRNAs in the crista neurosensory epithelium and vestibular ganglia following ototoxic damage

• Published in: Brain research Vol. 846; no. 1; pp. 40 - 51
• Main Authors: Popper, P., Lopez, I., Beizai, P., Li, G., Kim, J., Micevych, P.E., Honrubia, V.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 30-10-1999; Amsterdam Elsevier; New York, NY
• Subjects: aminoglycosides; Animals; BDNF; Biological and medical sciences; Brain-Derived Neurotrophic Factor - genetics; Chinchilla; crista; Epithelial Cells - cytology; Fundamental and applied biological sciences. Psychology; Gene Expression - physiology; Gentamicins - toxicity; In Situ Hybridization; Male; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration; Nerve Regeneration - physiology; Ototoxicity; Receptor, trkB - genetics; Regeneration; RNA, Messenger - analysis; Semicircular Canals - chemistry; Semicircular Canals - cytology; TrkB; Vertebrates: nervous system and sense organs; Vestibular epithelia; Vestibular Nerve - chemistry; Vestibular Nerve - cytology; Vestibulocochlear Nerve Diseases - chemically induced; Vestibulocochlear Nerve Diseases - physiopathology; Regeneration; TrkB; Vestibular epithelia; BDNF; Ototoxicity; Vestibular ganglion; Vertebrata; Mammalia; Toxicity; Gentamicin; Rodentia; Brain derived neurotrophic factor; Epithelium; Gene expression; Chinchilla
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(99)01941-1

Following ototoxic lesion with the aminoglycoside gentamicin, the vestibular neurosensory epithelia undergo degeneration and then limited spontaneous regeneration. The spatio-temporal expression of brain-derived neurotrophic factor (BDNF) and of its high affinity receptor (
trkB) mRNA was investigated in the vestibular end organs and ganglia of chinchillas following gentamicin ototoxicity. In the vestibular ganglia of untreated chinchillas, the level of expression of BDNF mRNA is low. At 1 and 2 weeks after intraotic treatment with gentamicin, BDNF mRNA levels in the vestibular ganglia were elevated significantly compared to untreated chinchillas and chinchillas 4 weeks after treatment. At 4 weeks after gentamicin treatment, BDNF mRNA levels were at intact levels of expression. In the crista ampullaris, high levels of BDNF transcripts were found in the untreated chinchillas. At 1 and 2 weeks after treatment, when only supporting cells are present in the crista, BDNF mRNA was undetectable. Four weeks after aminoglycoside treatment BDNF mRNA was present in the epithelium but at lower levels than in the intact epithelium. In contrast to its ligand, high levels of
trkB mRNA hybridization were present in the vestibular ganglia of untreated chinchillas and
trkB mRNA levels did not change following gentamicin treatment. In the vestibular epithelia,
trkB mRNA was not detected either in the intact epithelium or after gentamicin ototoxicity. These data suggest that BDNF may be involved in the maintenance of the vestibular ganglia and contribute to neurite outgrowth to new and repaired hair cells following ototoxic damage.