Clinical applications of metagenomic next-generation sequencing in the identification of pathogens in periprosthetic joint infections: a retrospective study

• Published in: Journal of orthopaedic surgery and research Vol. 19; no. 1; p. 301
• Main Authors: Shi, Tengfei, Chen, Huiyu, Liu, Yinhuan, Wu, Yexin, Lin, Feitai
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 17-05-2024; BioMed Central; BMC
• Subjects: Adult; Aged; Aged, 80 and over; Comparative analysis; Dermatologic agents; Dermatology; Female; Formulae, receipts, prescriptions; Health aspects; High-Throughput Nucleotide Sequencing - methods; Humans; Infection; Male; Medical research; Medicine, Experimental; Metagenomic next-generation sequencing; Metagenomics - methods; Microbial culture; Middle Aged; Pathogenic microorganisms; Periprosthetic joint infection; Predictive Value of Tests; Prosthesis-Related Infections - diagnosis; Prosthesis-Related Infections - microbiology; Retrospective Studies; Sensitivity and Specificity; Sinus tract; Staphylococcal infections; Staphylococcus aureus; Metagenomic next-generation sequencing; Periprosthetic joint infection; Microbial culture; Sinus tract; Staphylococcus aureus
• ISSN: 1749-799X; 1749-799X
• DOI: 10.1186/s13018-024-04745-5

This study aimed to evaluate the application of metagenomic next-generation sequencing (mNGS) technology to identify pathogens in periprosthetic joint infection (PJI).
A retrospective analysis was conducted on 65 patients suspected of having PJI between April 2020 and July 2023. The patients were categorized into PJI (46 patients) and non-PJI (19 patients) groups based on the 2018 International Consensus Meeting criteria. Clinical data were collected, and both conventional bacterial culture and mNGS were performed. The diagnostic performance of the two methods was compared and analyzed.
mNGS exhibited a sensitivity of 89.13%, a specificity of 94.74%, a positive predictive value of 97.62%, a negative predictive value of 78.26%, and an overall diagnostic accuracy of 90.77%. Compared to microbial culture, mNGS demonstrated superior diagnostic sensitivity while maintaining similar specificity. A total of 48 pathogens were successfully identified using mNGS, with Coagulase-negative staphylococci, Streptococci, Staphylococcus aureus, and Cutibacterium acnes being the most common infectious agents. Notably, mNGS was used to identify 17 potential pathogens in 14 culture-negative PJI samples, highlighting its ability to detect rare infectious agents, including Cutibacterium acnes (n = 5), Granulicatella adiacens (n = 1), Mycobacterium tuberculosis complex (n = 1), and Coxiella burnetii (n = 1), among others, which are not detectable by routine culture methods. However, mNGS failed to detect the pathogen in 4 culture-positive PJI patients, indicating its limitations. Among the 46 PJI patients, 27 had positive culture and mNGS results. The results of mNGS were concordant with those of culture at the genus level in 6 patients with PJI and at the species level in 18 patients. Furthermore, the present study revealed a significantly greater proportion of Staphylococcus aureus in the sinus tract group (45.45%) than in the non-sinus tract group (14.29%), indicating the association of this pathogen with sinus formation in PJI (P = 0.03). Additionally, there was no significant difference in the occurrence of polymicrobial infections between the sinus tract group (27.27%) and the non-sinus tract group (33.33%) (P = 0.37).
Metagenomic next-generation sequencing can serve as a valuable screening tool in addition to traditional culture methods to improve diagnostic accuracy through optimized culture strategies.