Toxicity of peritoneal dialysis fluids on cultured fibroblasts, L-929

• Published in: Kidney international Vol. 40; no. 1; pp. 77 - 79
• Main Authors: Wieslander, Anders P., Nordin, Marika K., Kjellstrand, Per T.T., Boberg, Ulf C.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-07-1991; Nature Publishing
• Subjects: Animals; Biological and medical sciences; Cell Division - drug effects; Cell Line; Dialysis Solutions - toxicity; Disinfection - methods; Drug toxicity and drugs side effects treatment; Fibroblasts - cytology; Fibroblasts - drug effects; Filtration; Medical sciences; Miscellaneous (drug allergy, mutagens, teratogens...); Peritoneal Dialysis; Pharmacology. Drug treatments; Cell culture; Toxicity; Dialysis fluid; Cytotoxicity; Fibroblast; Peritoneal dialysis; Peritoneum
• ISSN: 0085-2538; 1523-1755
• DOI: 10.1038/ki.1991.182

Toxicity of peritoneal dialysis fluids on cultured fibroblasts, L-929. Peritoneal dialysis (PD) fluids are known to suppress the reactions of inflammatory cells in vitro PD-fluids have also been shown to have cytotoxic influence on mesothelial cells. The combinations of these factors may have a detrimental effect on the peritoneum or may impair cellular defence against bacterial peritonitis. Some authors have discussed the relevance of heat sterilization to both so-called peritoneal side-effects and to chemical decomposition of fluids. Four commercial PD-fluids and one laboratory-made PD-fluid were tested for cytotoxicity on a cultured fibroblast cell line, L-929. Cytotoxicity was determined as an inhibition of cell growth by quantification of total protein. The laboratory-made PD-fluid was sterilized either by filtration or by filtration and heat. The commercial and the heat-sterilized laboratory made PD-fluids caused significant inhibition of cell growth (53 to 76%) in contrast to saline and the filter-sterilized laboratory-made PD-fluid. Since the pH values of all the testsolutions were neutral, low pH was not the cause of toxicity. Our results regarding the L-929 cells indicate that the cytotoxicity of PD-fluids is of a general nature. Furthermore, the results indicate that the heat sterilization process might be partially responsible for causing toxicity in PD-fluids.