Structure of a Human Gastrin Gene

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 4; pp. 1067 - 1069
• Main Authors: Wiborg, Ove, Berglund, Lars, Boel, Esper, Norris, Fanny, Norris, Kjeld, Rehfeld, Jens F., Marcker, Kjeld A., Vuust, Jens
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-02-1984; National Acad Sciences
• Subjects: Amino Acid Sequence; Base Composition; Base Sequence; Cloning, Molecular; Codon; Complementary DNA; DNA; DNA - analysis; DNA Restriction Enzymes; Gastrins - genetics; Generally accepted auditing standards; Genes; Genomics; Humans; Introns; Messenger RNA; Nucleic Acid Hybridization; Nucleotide sequences; Nucleotides; Start codon
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.81.4.1067

A gastrin gene was isolated from a genomic library of human DNA. The human gastrin gene is about 4100 base pairs long and contains two intervening sequences. Thus, a 3500-base-pair intervening sequence is located 5 base pairs proximal to the ATG initiator codon, while a 129-base-pair intervening sequence separates the region coding for the principal hormonal form of gastrin, the heptadecapeptide, from the region coding for the major amino-terminal portion of the gastrin precursor. The 5′flanking region of the gene contains the conserved sequences, T-A-T-A-A and G-A-C-T-C-A-T-A-T, in positions similar to those of other eukaryotic genes.