Comparison of two molecular techniques for the detection of avian reoviruses in formalin-fixed, paraffin-embedded chicken tissues

• Published in: Journal of virological methods Vol. 80; no. 2; pp. 197 - 201
• Main Authors: Liu, Hung J., Liao, Ming H., Chang, C.D., Chen, Jen H., Lin, Maw Y., Tung, Ming C.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01-07-1999; Amsterdam Elsevier; New York, NY
• Subjects: AIDS/HIV; Animal viral diseases; Animals; Avian reovirus; Biological and medical sciences; Chickens - virology; DNA, Viral - analysis; Fundamental and applied biological sciences. Psychology; In situ hybridization (ISH); In Situ Hybridization - veterinary; Infectious diseases; Medical sciences; Microbiology; Molecular Probe Techniques - veterinary; Orthoreovirus - genetics; Orthoreovirus - isolation & purification; Paraffin Embedding - veterinary; Reoviridae Infections - veterinary; Reoviridae Infections - virology; Reverse Transcriptase Polymerase Chain Reaction - veterinary; Reverse transcription (RT) in situ polymerase chain reaction (PCR); Techniques used in virology; Tissue Embedding - veterinary; Viral diseases; Virology; In situ hybridization (ISH); Avian reovirus; Reverse transcription (RT) in situ polymerase chain reaction (PCR); Performance evaluation; Molecular hybridization; RNA; In situ; Method; Paraffin; Reoviridae; Formaldehyde; Infection; Virus; Polymerase chain reaction; Vertebrata; Sample preparation; Animal; Viral disease; Orthoreovirus; Chicken; Diagnosis; Aves; Veterinary; Detection
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(99)00047-6

Reverse transcription (RT) in situ polymerase chain reaction (PCR) and in situ hybridization (ISH) techniques were used to detect the σc-encoded gene of avian reovirus (ARV) in chicken tissue sections. The advantage of using in situ methods is to make more rapid and accurate diagnosis of ARV infections. The sensitivity of these two techniques were compared. Of the two techniques, the RT in situ PCR test was found to be more sensitive than ISH and provided the rapid, sensitive, and specific detection of ARV infections.