Evaluation of autofocus functions in molecular cytogenetic analysis

This work describes a systematic evaluation of several autofocus functions used for analytical fluorescent image cytometry studies of counterstained nuclei. Focusing is the first step in the automatic fluorescence in situ hybridization analysis of cells. Thirteen functions have been evaluated using...

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Bibliographic Details
Published in:	Journal of microscopy (Oxford) Vol. 188; no. 3; pp. 264 - 272
Main Authors:	SANTOS, A., ORTIZ DE SOLÓRZANO, C., VAQUERO, J. J., PEÑA, J. M., MALPICA, N., DEL POZO, F.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-12-1997
Subjects:	Algorithms Analytical cytometry autofocus Blood Cells - cytology Bone Marrow Cells - cytology Cell Nucleus Cytogenetics - methods FISH Flow Cytometry focusing algorithms Humans Image Processing, Computer-Assisted In Situ Hybridization
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Summary:	This work describes a systematic evaluation of several autofocus functions used for analytical fluorescent image cytometry studies of counterstained nuclei. Focusing is the first step in the automatic fluorescence in situ hybridization analysis of cells. Thirteen functions have been evaluated using qualitative and quantitative procedures. For the last of these procedures a figure‐of‐merit (FOM) is defined and proposed. This new FOM takes into account five important features of the focusing function. Our results show that functions based on correlation measures have the best performance for this type of image.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-2720 1365-2818
DOI:	10.1046/j.1365-2818.1997.2630819.x