codominant SCAR marker linked to the genic male sterility gene (ms₁) in chili pepper (Capsicum annuum)

• Published in: Plant breeding Vol. 129; no. 1; pp. 35 - 38
• Main Authors: Lee, J, Yoon, J.B, Han, J.-H, Lee, W.P, Do, J.W, Ryu, H, Kim, S.H, Park, H.G
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-02-2010; Blackwell Publishing Ltd; Blackwell
• Subjects: AFLP-SCAR; Agronomy. Soil science and plant productions; Biological and medical sciences; Capsicum annuum; chili pepper; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; genic male sterility; Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia; ms; ms 1; Plant breeding: fundamental aspects and methodology; Sequence characterized amplified region marker; ms; Vegetable crop; Gene; AFLP-SCAR; Dicotyledones; Angiospermae; Genetic improvement; Flavoring crop; Capsicum annuum; Genic male sterility; Spermatophyta; Solanaceae; Amplified fragment length polymorphism marker; Chilli pepper; chili pepper
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2009.01643.x

With 3 figures As one of the genic male sterility (GMS) materials in chili pepper (Capsicum annuum L.), GMS1 has been used for commercial F₁ hybrid seed production. The male sterility of GMS1 is controlled by a recessive nuclear gene, named ms₁. In this study, we developed DNA markers linked to the ms₁ locus using a combination of bulked segregant analysis and amplified fragment length polymorphism (AFLP) in a segregating sibling population. From the screening of 1024 AFLP primer combinations, the AFLP marker E-AGC/M-GTG (514 bp) was identified as being linked to the ms₁ locus at a distance of about 3 cM. Based on internal sequencing analysis of the E-AGC/M-GTG marker between male fertile and sterile plants, we identified three small deletions with a size of altogether 42 bp in the male-fertile plant and developed a codominant sequence characterized amplified region (SCAR) marker. This SCAR marker may be valuable for marker-assisted breeding in the hybrid seed production system of chili pepper using the GMS1 line.