Headspace solid-phase microextraction and capillary gas chromatographic-mass spectrometric determination of rivastigmine in canine plasma samples

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 806; no. 2; pp. 271 - 276
• Main Authors: Sha, Yunfei, Deng, Chunhui, Liu, Zhen, Huang, Taomin, Yang, Bei, Duan, Gengli
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05-07-2004; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cholinesterase Inhibitors - blood; Dogs; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry - methods; General pharmacology; Medical sciences; Neuroprotective Agents - blood; Pharmacology. Drug treatments; Phenylcarbamates - blood; Rivastigmine; Rivastigmine; Biological fluid; Temperature; Psychotropic; Esterases; Acetylcholinesterase; Blood plasma; Spectrometry; Investigation method; Anticholinesterase agent; Dog; Quantitative analysis; Fissipedia; Carnivora; Enzyme; Single dose; Oral administration; Enzyme inhibitor; Antialzheimer agent; Carboxylic ester hydrolases; Solid phase microextraction; Gas chromatography; Vertebrata; Mammalia; Sodium; Animal; Hydrolases; Headspace
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2004.04.006

A simple, rapid and sensitive method for determination of rivastigmine in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC–MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-μm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5
ml of plasma modified with 1.0
ml of sodium hydroxide-sodium carbonate solution (0.7
M:0.5
M); extraction temperature of 100
°C, with stirring at 2000
rpm for 30
min. The calibration curve showed linearity in the range from 0.2 to 80
ng/ml with regression coefficient corresponding to 0.9965 and coefficient of the variation of the points of the calibration curve lower than 10%. The quantification limit for rivastigmine in plasma was 0.2
ng/ml. The method was applied to determination of rivastigmine in canine plasma samples from animals after a single oral administration.