Serum matrix metalloproteinase-7 levels in infants with cholestasis and biliary atresia

• Published in: BMC pediatrics Vol. 22; no. 1; pp. 1 - 351
• Main Authors: Rohani, Pejman, Mirrahimi, Seyyed Bahador, Bashirirad, Haleh, Rahmani, Parisa, Kamran, Niyoosha, Alimadadi, Hosein, Hajipour, Mahmoud, Sohouli, Mohammad Hassan
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 18-06-2022; BioMed Central; BMC
• Subjects: Age; Alkaline phosphatase; Babies; Biliary atresia; Biomarkers; Care and treatment; Children & youth; Cholestasis; Congenital diseases; Cytomegalovirus; Diagnosis; Disease; Enzymes; Gallbladder; Gallbladder diseases; GGT; Health aspects; Hospitals; Infant health services; Jaundice, Obstructive; Liver; Measurement; MMP7; Pediatrics; Risk factors; Transplants & implants; Variance analysis; Iran
• ISSN: 1471-2431; 1471-2431
• DOI: 10.1186/s12887-022-03409-9

Background The aim of this study was to evaluate the serum level of matrix metalloproteinase 7 (MMP7) in infants with cholestasis and the diagnostic values of this biomarker to differentiate biliary atresia (BA) from other causes of cholestasis. Methods This multi-center study is conducted during 2 years in Mofid children's hospital and Children's Medical Center, Pediatrics Center of Excellence Tehran, Iran. 54 infants with cholestasis were enrolled in this study with a control group consists of 41 healthy infants with the same age. Serum samples were taken from all these patients to assess serum levels of MMP7, Gamma-glutamyl Transferase (GGT). For each biomarker, we calculated the sensitivity and specificity and other statistical characteristics. Results There were 89 subjects, 22 patients with BA, 32 patients with non-BA cholestasis and 41 subjects as control group. The mean serum MMP7 levels in BA, non-BA cholestasis and control group was 15.91 ng/ml [+ or -] 6.64, 4.73 ng/ml [+ or -] 2.59 and 0.49 ng/ml [+ or -] 0.33, respectively. The best cut-off point is calculated 7.8 ng/ml for MMP7 and 434.5 U/L for GGT. The area under curve (AUC) for these two markers are 0.988 [+ or -] 0.008 and 0.854 [+ or -] 0.052, respectively. The sensitivity and specificity of MMP7 to differentiate biliary atresia from nonbiliary atresia cholestasis in our study was 95.5% and 94.5%, respectively. The sensitivity and specificity of GGT was 77.3% and 77.8%, respectively. These results show that the MMP7 has more sensitivity and specificity in differentiation. Conclusion MMP7 demonstrated good accuracy to differentiate biliary atresia from other causes of cholestasis. Keywords: MMP7, GGT, Biliary atresia, Alkaline phosphatase