Effect of sucralfate on gastric permeability in an ex vivo model of stress‐related mucosal disease in dogs

• Published in: Journal of veterinary internal medicine Vol. 32; no. 2; pp. 670 - 678
• Main Authors: Hill, Tracy L., Lascelles, B. Duncan X., Blikslager, Anthony T.
• Format: Journal Article
• Language: English
• Published: United States John Wiley and Sons Inc 01-03-2018; Wiley
• Subjects: Animals; Anti-Ulcer Agents - administration & dosage; Anti-Ulcer Agents - pharmacology; barrier function; Dog Diseases - chemically induced; Dog Diseases - drug therapy; Dog Diseases - prevention & control; Dogs; Gastric Mucosa - drug effects; Gastric Mucosa - pathology; Hydrogen-Ion Concentration; In Vitro Techniques; Isotonic Solutions; Ringer's Solution; SMALL ANIMAL; Stomach Ulcer - chemically induced; Stomach Ulcer - drug therapy; Stomach Ulcer - prevention & control; Stomach Ulcer - veterinary; stress ulcer; Sucralfate - administration & dosage; Sucralfate - pharmacology; transepithelial electrical resistance; Ussing chamber; Ussing chamber; stress ulcer; transepithelial electrical resistance; barrier function
• ISSN: 0891-6640; 1939-1676
• DOI: 10.1111/jvim.15076

Background
Sucralfate is a gastroprotectant with no known systemic effects. The efficacy of sucralfate for prevention and treatment of stress‐related mucosal diseases (SRMD) in dogs is unknown.
Hypothesis/Objectives
To develop a canine ex vivo model of SRMD and to determine the effect of sucralfate on mucosal barrier function in this model.
Animals
Gastric antral mucosa was collected immediately postmortem from 29 random‐source apparently healthy dogs euthanized at a local animal control facility.
Methods
Randomized experimental trial. Sucralfate (100 mg/mL) was applied to ex vivo canine gastric mucosa concurrent with and after acid injury. Barrier function was assessed by measurement of transepithelial electrical resistance (TER) and radiolabeled mannitol flux.
Results
Application of acidified Ringers solution to the mucosal side of gastric antrum caused a reduction in gastric barrier function, and washout of acidified Ringers solution allowed recovery of barrier function (TER: 34.0 ± 2.8% of control at maximum injury, 71.3 ± 5.5% at recovery, P < .001). Sucralfate application at the time of injury or after injury significantly hastened recovery of barrier function (TER: 118.0 ± 15.2% of control at maximum injury, P < .001 and 111.0 ± 15.5% at recovery, P = .35).
Conclusions and Clinical Importance
Sucralfate appeared effective at restoring defects in gastric barrier function induced by acid and accelerating repair of tissues subjected to acid in this model, suggesting that sucralfate could have utility for the treatment and prevention of SRMD in dogs.