Complete genome sequence of a new botybirnavirus isolated from a phytopathogenic Alternaria alternata in China

• Published in: Archives of virology Vol. 164; no. 4; pp. 1225 - 1228
• Main Authors: Ma, Guoping, Liang, Zhijian, Hua, Huihui, Zhou, Tao, Wu, Xuehong
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-04-2019; Springer Nature B.V
• Subjects: Alternaria alternata; Amino acids; Annotated Sequence Record; Biomedical and Life Sciences; Biomedicine; DNA-directed RNA polymerase; Double-stranded RNA; Fusion protein; Genomes; Infectious Diseases; Isometric; Medical Microbiology; Nucleotide sequence; Open reading frames; Phylogeny; Phytopathogenic fungi; Polyproteins; RNA polymerase; RNA viruses; Virology; China
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-019-04189-x

A new double-stranded RNA (dsRNA) virus named Alternaria alternata botybirnavirus 1 (AaBRV1) was isolated from
Alternaria alternata
strain SD-BZF-19, a phytopathogenic fungus infecting watermelon in China. The genome of AaBRV1 consists of two dsRNA segments (dsRNAs 1 and 2), 6,130 and 5,862 bp in size, respectively. The sequence contains two putative open reading frames (ORFs) which encode two polyproteins of 1,874 and 1,784 amino acids, respectively. Nucleotide sequence comparisons revealed that the two ORFs of AaBRV1 have the highest similarity 60.3% and 56.7%, respectively, with dsRNAs 1 and 2 of
Botrytis porri RNA virus 1
(BpRV1). The two polyproteins encoded by dsRNA1 and dsRNA2 shared the highest amino acid identities with the cap-pol fusion protein (60.2%) and hypothetical protein (53.7%) of BpRV1, respectively. AaBRV1 is composed of isometric particles, approximately 35 nm in diameter. Phylogenetic analysis of the RNA dependent RNA polymerase (RdRp) domain of the polyprotein revealed that AaBRV1 clusters together with members of the genus
Botybirnavirus
. These findings support the discovery of a new botybirnavirus in
A. alternata
.