Projective light-sheet microscopy with flexible parameter selection

• Published in: Nature communications Vol. 15; no. 1; pp. 2755 - 8
• Main Authors: Chen, Bingying, Chang, Bo-Jui, Daetwyler, Stephan, Zhou, Felix, Sharma, Shiv, Lee, Donghoon M., Nayak, Amruta, Noh, Jungsik, Dubrovinski, Konstantin, Chen, Elizabeth H., Glotzer, Michael, Fiolka, Reto
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29-03-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 14/63; 631/1647/245/2225; 631/1647/245/2226; 631/1647/767/1658; Animals; Brain - ultrastructure; Cellular structure; Drosophila; Fluorescence; Fluorescence microscopy; Fruit flies; Humanities and Social Sciences; Image contrast; Image resolution; Insects; Interrogation; Larva; Larvae; Light sheets; Microscopy; Microscopy, Fluorescence - methods; multidisciplinary; Neuroimaging; Parameters; Science; Science (multidisciplinary); Viewing; Zebrafish
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-46693-y

Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up. Here, we demonstrate rapid
pro
jective light-sheet imaging with
p
arameter
s
election (props) of imaging depth, position and viewing angle. This allows us to selectively image different sub-volumes of a sample, rapidly switch between them and exclude background fluorescence. Here we demonstrate the power of props by functional imaging within distinct regions of the zebrafish brain, monitoring calcium firing inside muscle cells of moving Drosophila larvae, super-resolution imaging of selected cell layers, and by optically unwrapping the curved surface of a Drosophila embryo. We anticipate that props will accelerate volumetric interrogation, ranging from subcellular to mesoscopic scales.
Projection imaging for multi-cellular samples can be hindered by several factors, including low contrast. Here, the authors propose projective light-sheet imaging with parameter selection (props) of imaging depth, position and viewing angle.