Autohydrolysis Application on Vine Shoots and Grape Stalks to Obtain Extracts Enriched in Xylo-Oligosaccharides and Phenolic Compounds

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 9; p. 3760
• Main Authors: Difonzo, Graziana, Troilo, Marica, Casiello, Michele, D'Accolti, Lucia, Caponio, Francesco
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-04-2023; MDPI
• Subjects: Agricultural practices; antioxidant; Antioxidants - pharmacology; by-products; Cellulose; Colon; Environmental effects; Ethanol; Food; Grapes; Hydrolysis; Lignocellulose; Monosaccharides; Oligosaccharides; Oligosaccharides - chemistry; Phenolic compounds; Phenols; Plant Extracts - chemistry; Polyphenols; Prebiotics; Quantitative analysis; Solvents; Sugar; Sugars; Sustainability; Vitis - chemistry; winemaking; Xylan; xylo-oligosaccharides; Germany; winemaking; prebiotics; antioxidant; by-products; xylo-oligosaccharides
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28093760

Agronomic practices and the winemaking process lead to the production of considerable quantities of waste and by-products. These are often considered waste with negative effects on environmental sustainability. However, vine shoots and grape stalks can be reused, representing a potential source of xylo-oligosaccharides and polyphenols. In this context, the purpose of this work was to obtain enriched extracts using three different autohydrolysis treatments with (i) H
O, (ii) H
O:EtOH, and (iii) H
O:Amberlyst. The obtained extracts were characterized by their xylo-oligosaccharide and polyphenol profiles using LC-MS techniques. The use of ethanol during autohydrolysis allowed for greater extraction of xylan-class compounds, especially in vine shoot samples, while an increase in antioxidant activity (128.04 and 425.66 µmol TE/g for ABTS and DPPH, respectively) and in total phenol content (90.92 mg GAE/g) was obtained for grape stalks.