Erythroprotective Potential of Phycobiliproteins Extracted from Porphyridium cruentum

• Published in: Metabolites Vol. 13; no. 3; p. 366
• Main Authors: Peña-Medina, Rubria Lucía, Fimbres-Olivarría, Diana, Enríquez-Ocaña, Luis Fernando, Martínez-Córdova, Luis Rafael, Del-Toro-Sánchez, Carmen Lizette, López-Elías, José Antonio, González-Vega, Ricardo Iván
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-03-2023; MDPI
• Subjects: ABO system; Algae; Antigens; antihemolytic; Antioxidants; Aquatic microorganisms; Bioactive compounds; Blood diseases; Blood groups; Breast cancer; Carcinoma; Coronaviruses; COVID-19; Electron transfer; erythroprotective effect; Free radicals; Gallstones; Hemolysis; Hypertension; Hypotonicity; ISO standards; Laboratories; Lipid peroxidation; microalgae; Oxidation; Oxidative stress; Pancreatic cancer; Phycobiliproteins; pigment-rich extracts; Pigments; Porphyridium cruentum; red blood cells; Spectrophotometry; Thrombosis; Thyroid cancer; Ulcers; red blood cells; microalgae; pigment-rich extracts; antihemolytic; erythroprotective effect; phycobiliproteins
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo13030366

There are multiple associations between the different blood groups (ABO and RhD) and the incidence of oxidative stress-related diseases, such as certain carcinomas and COVID-19. Bioactive compounds represent an alternative to its prevention and treatment. Phycobiliproteins (PBP) are bioactive compounds present in the microalga
and, despite its antioxidant activity, their inhibitory effect on hemolysis has not been reported. The aim of this work was to evaluate the erythroprotective potential of phycobiliproteins from
in different blood groups. The microalga was cultured in F/2 medium under controlled laboratory conditions. Day 10 of culture was determined as the harvest point. The microalgal biomass was lyophilized and a methanolic (MetOH), Tris HCl (T-HCl), and a physiological solution (PS) ultrasound-assisted extraction were performed. Extract pigments were quantified by spectrophotometry. The antioxidant activity of the extracts was evaluated with the ABTS
, DPPH
, and FRAP methods, finding that the main antioxidant mechanism on the aqueous extracts was HAT (hydrogen atom transfer), while for MetOH it was SET (single electron transfer). The results of the AAPH, hypotonicity, and heat-induced hemolysis revealed a probable relationship between the different antigens (ABO and RhD) with the antihemolytic effect, highlighting the importance of bio-directed drugs.