Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells

• Published in: Antioxidants Vol. 12; no. 4; p. 914
• Main Authors: Ren, Heng, Li, Kejie, Min, Yan, Qiu, Binhang, Huang, Xiaolu, Luo, Jingxin, Qi, Liwen, Kang, Maoli, Xia, Peng, Qiao, Hanzhen, Chen, Jun, Cui, Yaoming, Gan, Liping, Wang, Peng, Wang, Jinrong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12-04-2023; MDPI
• Subjects: anti-inflammatory; antioxidant; Antioxidants; Decolorization; Epithelial cells; Hydrogen peroxide; Inflammation; Methods; NF-κB protein; Optimization; Pathogens; pathways; Polysaccharides; Rehmannia glutinosa; Rehmannia glutinosa polysaccharides; Sulfuric acid; TLR4 protein; Toll-like receptors; China; anti-inflammatory; antioxidant; pathways; decolorization; Rehmannia glutinosa polysaccharides
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox12040914

Polysaccharide decolorization has a major effect on polysaccharide function. In the present study, the decolorization of
polysaccharides (RGP) is optimized using two methods-the AB-8 macroporous resin (RGP-1) method and the H
O
(RGP-2) method. The optimal decolorization parameters for the AB-8 macroporous resin method were as follows: temperature, 50 °C; macroporous resin addition, 8.4%; decolorization duration, 64 min; and pH, 5. Under these conditions, the overall score was 65.29 ± 3.4%. The optimal decolorization conditions for the H
O
method were as follows: temperature, 51 °C; H
O
addition, 9.5%; decolorization duration, 2 h; and pH, 8.6. Under these conditions, the overall score was 79.29 ± 4.8%. Two pure polysaccharides (RGP-1-A and RGP-2-A) were isolated from RGP-1 and RGP-2. Subsequently, their antioxidant and anti-inflammatory effects and mechanisms were evaluated. RGP treatment activated the Nrf2/Keap1 pathway and significantly increased the activity of antioxidant enzymes (
< 0.05). It also inhibited the expression of pro-inflammatory factors and suppressed the TLR4/NF-κB pathway (
< 0.05). RGP-1-A had a significantly better protective effect than RGP-2-A, likely owing to the sulfate and uronic groups it contains. Together, the findings indicate that RGP can act as a natural agent for the prevention of oxidation and inflammation-related diseases.