Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase

• Published in: Journal of bioscience and bioengineering Vol. 117; no. 6; pp. 676 - 679
• Main Authors: Kojima, Yusuke, Wakita, Jun, Inayoshi, Yujin, Suzuki, Ryosuke, Yamada, Yuki, Kaneoka, Hidenori, Nishijima, Ken-ichi, Iijima, Shinji
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-06-2014; Elsevier
• Subjects: Animals; Animals, Genetically Modified - genetics; Avian Proteins - biosynthesis; Avian Proteins - genetics; Biological and medical sciences; Biotechnology; Chick Embryo; Chickens - genetics; Egg Proteins - metabolism; Erythropoietin; Erythropoietin - genetics; Erythropoietin - metabolism; Fundamental and applied biological sciences. Psychology; Galactose; Galactose - metabolism; Galactosyltransferase; Galactosyltransferases - biosynthesis; Galactosyltransferases - genetics; Glycosylation; Humans; N-Glycan; Plasmids; Protein Processing, Post-Translational; Transgenic chicken; Vesiculovirus - genetics; Transgenic chicken; Galactosyltransferase; Erythropoietin; Galactose; N-Glycan; Human; Enzyme; Transferases; Glycosyltransferases; Glycan; N-Acetyllactosaminide α-1,3-galactosyltransferase; Vertebrata; Hexosyltransferases; Chicken; Aves; Genetically modified organism; Transgenesis
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2013.11.010

Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated.