Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system al...

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Bibliographic Details
Published in:Lab on a chip Vol. 14; no. 23; p. 4455
Main Authors: Syed, A, Mangano, L, Mao, P, Han, J, Song, Y-A
Format: Journal Article
Language:English
Published: England 07-12-2014
Subjects:
Colloids - chemistry
Dimethylpolysiloxanes - chemistry
DNA - chemistry
DNA - isolation & purification
Equipment Design
Microfluidic Analytical Techniques - instrumentation
Nanostructures - chemistry
Nylons - chemistry
Proteins - chemistry
Proteins - isolation & purification
Silicon Dioxide - chemistry
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Summary:In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes.
ISSN:1473-0189
DOI:10.1039/c4lc00895b