Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing

• Published in: Journal of applied microbiology Vol. 114; no. 6; pp. 1713 - 1724
• Main Authors: Tall, A., Hervio‐Heath, D., Teillon, A., Boisset‐Helbert, C., Delesmont, R., Bodilis, J., Touron‐Bodilis, A.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01-06-2013; Oxford University Press; Wiley
• Subjects: Biodiversity; Biological and medical sciences; coastal environment; culturable Vibrio spp; diversity; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Life Sciences; Microbiology; Ocean temperature; Pathology; pyrH and toxR genes partial sequencing; Seawater - microbiology; Sequence Analysis, DNA; Temperature; Vibrio - classification; Vibrio - genetics; Vibrio - isolation & purification; Vibrio alginolyticus; Vibrio alginolyticus - isolation & purification; Vibrio parahaemolyticus; English Channel; Atlantic Ocean; North Atlantic; ANE, Europe, English Channel; Temperature; Gene; culturable Vibrio spp; Applied microbiology; Diversity; Bacteria; Vibrionaceae; Coastal environment; Sequencing; pyrH and toxR genes partial sequencing; Vibrio
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12181

Aims
To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments.
Methods and Results
q‐PCR assays previously selected for the identification of bacterial strains isolated at 37°C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22°C. The great majority of the 37°C strains was identified by q‐PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85·9%) and V. harveyi (10·7%). The human pathogens V. parahaemolyticus and V. cholerae were rarely detected (two strains each). The 22°C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V. celticus (36·8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22°C (80%) and 37°C (99·5%), respectively.
Conclusions
The combination of q‐PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37°C both dominated by pathogenic species for marine organisms.
Significance and Impact of the Study
The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22°C, as 92·3% of them were identified in this study.