Reproductive Effects of S. boulardii on Sub-Chronic Acetamiprid and Imidacloprid Toxicity in Male Rats

• Published in: Toxics (Basel) Vol. 11; no. 2; p. 170
• Main Authors: Sevim, Çiğdem, Akpınar, Erol, Aksu, Emrah Hicazi, Ömür, Ali Doğan, Yıldırım, Serkan, Kara, Mehtap, Bolat, İsmail, Tsatsakis, Aristides, Mesnage, Robin, Golokhvast, Kirill S, Uzunçakmak, Sevgi Karabulut, Ersoylu, Rabia Nilüfer
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12-02-2023; MDPI
• Subjects: acetamiprid; Animals; Apoptosis; Bacteria; Biocompatibility; Caspase-3; Connective tissue; Degeneration; Deoxyguanosine; Edema; Fungi; Gametocytes; Health promotion; Hyperemia; Imidacloprid; In vivo methods and tests; Insecticides; Intestinal microflora; Intoxication; Male reproductive system; Males; Metabolism; Microbiomes; Microbiota; Molecular modelling; Morphology; Motility; Necrosis; Ovaries; Oxidative stress; Pesticides; probiotic; Probiotics; Prostate; Reproductive system; Saccharomyces boulardii; Sperm; Spermatocytes; Testes; Toxicity; Saccharomyces boulardii; probiotic; acetamiprid; imidacloprid
• ISSN: 2305-6304; 2305-6304
• DOI: 10.3390/toxics11020170

The potential health-promoting effects of probiotics against intoxication by pesticides is a topic of increasing commercial interest with limited scientific evidence. In this study, we aimed to investigate the positive effects of probiotic
on the male reproductive system under low dose neonicotinoid pesticide exposure conditions. We observed that acetamiprid and imidacloprid caused a degeneration and necrosis of the spermatocytes in the tubular wall, a severe edema of the intertubular region and a hyperemia. This was concomittant to increased levels of 8-hydroxy-2'-deoxyguanosine reflecting oxidative stress, and an increase in caspase 3 expression, reflecting apoptosis. According to our results,
supplementation mitigates these toxic effects. Further in vivo and clinical studies are needed to clarify the molecular mechanisms of protection. Altogether, our study reinforces the burden of evidence from emerging studies linking the composition of the gut microbiome to the function of the reproductive system.