Screening of Croatian Native Grapevine Varieties for Susceptibility to Plasmopara viticola Using Leaf Disc Bioassay, Chlorophyll Fluorescence, and Multispectral Imaging

• Published in: Plants (Basel) Vol. 10; no. 4; p. 661
• Main Authors: Štambuk, Petra, Šikuten, Iva, Preiner, Darko, Nimac, Ana, Lazarević, Boris, Marković, Zvjezdana, Maletić, Edi, Kontić, Jasminka Karoglan, Tomaz, Ivana
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30-03-2021; MDPI
• Subjects: Airborne microorganisms; Bioassays; biotic stress; Chlorophyll; chlorophyll fluorescence; Cultivars; Cultivation; Downy mildew; Fluorescence; Fruit cultivation; Germplasm; Grapevines; imaging methodology; Infections; Inoculation; Leaves; Metabolism; Phenotyping; Photochemistry; Plant diseases; Plasmopara viticola; spectral indices; Sporulation; Vegetation; Vitis vinifera; Vitis vinifera L; Wines; Vitis vinifera L; phenotyping model; biotic stress; chlorophyll fluorescence; downy mildew; imaging methodology; spectral indices
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants10040661

In the era of sustainable grapevine production, there is a growing demand to define differences between
varieties in susceptibility to downy mildew. Croatia, as a country with a long tradition of grapevine cultivation, preserves a large number of native grapevine varieties. A leaf disc bioassay has been conducted on 25 of them to define their response to downy mildew, according to the International Organisation of Vine and Wine (OIV) descriptor 452-1, together with the stress response of the leaf discs using chlorophyll fluorescence and multispectral imaging with 11 parameters included. Time points of measurement were as follows: before treatment (T
), one day post-inoculation (dpi) (T
), two dpi (T
), three dpi (T
), four dpi (T
), six dpi (T
), and eight dpi (T
). Visible changes in form of developed
(
) sporulation were evaluated on the seventh day upon inoculation. Results show that methods applied here distinguish varieties of different responses to downy mildew. Based on the results obtained, a phenotyping model in the absence of the pathogen is proposed, which is required to confirm by conducting more extensive research.