Methodology for health risk assessment of combined exposures to multiple chemicals

• Published in: Food and chemical toxicology Vol. 143; p. 111520
• Main Authors: Beronius, Anna, Zilliacus, Johanna, Hanberg, Annika, Luijten, Mirjam, van der Voet, Hilko, van Klaveren, Jacob
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-09-2020
• Subjects: Adverse outcome pathway; Combined exposure; Computer Simulation; Environmental Exposure; Hazardous Substances - toxicity; Humans; Medicin och hälsovetenskap; Mixture; Models, Biological; Risk Assessment; Combined exposure; Risk assessment; Mixture; Adverse outcome pathway
• ISSN: 0278-6915; 1873-6351; 1873-6351
• DOI: 10.1016/j.fct.2020.111520

Focus on risks to human health and the environment from combined exposure to multiple chemicals (“mixture risk assessment”) has increased in the last couple of decades. There has been a rise in awareness and concern in the community, especially concerning unintentional environmental exposure to unknown chemical mixtures. The Horizon 2020 project EuroMix has developed methodologies and tools for mixture risk assessment with a focus on component-based approach where substances are grouped based on toxicological considerations. Dose addition is used as the model for calculating the combined toxicity of mixture components. The methodology is anchored in the Adverse Outcome Pathway (AOP) concept, which provides a structured basis for e.g. grouping substances into assessment groups and identifying and collecting relevant toxicity data. The aim of this paper is to describes development of the methodology for mixture risk assessment and to provide detailed methodology for problem formulation, use of AOP networks for development of tiered testing strategies and grouping of substances, as well as considerations for use of dose addition methodology.
•Methodologies and tools for mixture risk assessment (RA) have been developed in the EuroMix project.•The EuroMix methodology is anchored in the Adverse Outcome Pathway (AOP) concept.•It includes methods for problem formulation, development of tiered testing strategies and grouping of substances.•It includes considerations for use of dose addition methodology for mixture risk assessment.•The EuroMix methodologies and tools offer practical steps forward for mixture RA in line with guidance from OECD and EFSA.