Three new species of blood flukes (Digenea: Aporocotylidae) infecting pufferfishes (Teleostei: Tetraodontidae) from off Bali, Indonesia

• Published in: Parasitology international Vol. 65; no. 5; pp. 432 - 443
• Main Authors: Yong, R.Q.-Y, Cutmore, S.C, Bray, R.A, Miller, T.L, Semarariana, I.W.Y, Palm, H.W, Cribb, T.H
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01-10-2016
• Subjects: Animals; Blood flukes; Coral Triangle; Degenerate posterior testes; DNA, Ribosomal Spacer - genetics; Female; Fish Diseases - parasitology; Gastroenterology and Hepatology; Indonesia; Infectious Disease; Male; Molecular Typing; Phylogeny; Psettarium; RNA, Ribosomal, 28S - genetics; Schistosomatidae - anatomy & histology; Schistosomatidae - classification; Schistosomatidae - genetics; Schistosomatidae - isolation & purification; Speciation; Tetraodontiformes - parasitology; Trematode Infections - parasitology; Trematode Infections - veterinary; Blood flukes; Coral triangle; Speciation; Degenerate posterior testes; Psettarium; Coral Triangle
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2016.05.011

Abstract We describe three new species of blood flukes (Aporocotylidae) and propose their classification within the genus Psettarium Goto & Ozaki, 1929. All three species were collected from the circulatory systems of pufferfishes caught off Bali, central Indonesia. Psettarium pulchellum n. sp. was found in the gills of both the narrow-lined puffer ( Arothron manilensis de Procé) and the spiny blaasop ( Tylerius spinosissimus Regan), while Papillacarus ogawai n. sp. and P. jimbaranensis n. sp. were found in the gills of the reticulated puffer ( Arothron reticularis Bloch & Schneider). The morphological characteristics of these taxa necessitated emendation of the diagnosis for the genus Psettarium , to accommodate the presence of an oral sucker, multiple or entirely post-caecal testes and a degenerate posterior testis. Features such as proportion of body length occupied by the oesophagus, and posterior caeca being ≥ 7 × the length of anterior caeca, are no longer regarded as useful genus-level characters. Additionally, Sasala nolani is reassigned to this genus as Psettarium nolani n. comb. In phylogenetic analyses of the 28S and ITS2 rDNA regions, all three new taxa form a well-supported clade, together with Psettarium sinense and Psettarium nolani n. comb., the two other species of tetraodontid-infecting aporocotylids for which comparative rDNA data were available. The short branch lengths within this clade, despite dramatic morphological differences between the five species, suggest that rapid morphological diversification has occurred among the tetraodontid-infecting aporocotylids. The genus Psettarium has long been considered problematic. Further commentary is given on the history of this genus and how the issues presented might be resolved.