Peroxiredoxin 5 confers protection against oxidative stress and apoptosis and also promotes longevity in Drosophila

• Published in: Biochemical journal Vol. 419; no. 2; p. 437
• Main Authors: Radyuk, Svetlana N, Michalak, Katarzyna, Klichko, Vladimir I, Benes, Judith, Rebrin, Igor, Sohal, Rajindar S, Orr, William C
• Format: Journal Article
• Language: English
• Published: England 15-04-2009
• Subjects: Animals; Apoptosis - genetics; Apoptosis - physiology; Cell Line; Chromatography, High Pressure Liquid; Drosophila; Flow Cytometry; Gene Expression Regulation, Developmental; Immunoblotting; In Situ Nick-End Labeling; Longevity - genetics; Longevity - physiology; Mutation; Oxidative Stress - genetics; Oxidative Stress - physiology; Peroxiredoxins - genetics; Peroxiredoxins - metabolism; Peroxiredoxins - physiology; Phenotype
• ISSN: 1470-8728
• DOI: 10.1042/bj20082003

Peroxiredoxin 5 is a distinct isoform of the peroxiredoxin gene family. The antioxidative and anti-apoptotic functions of peroxiredoxin 5 have been extensively demonstrated in cell culture experiments. In the present paper, we provide the first functional analysis of peroxiredoxin 5 in a multicellular organism, Drosophila melanogaster. Similar to its mammalian, yeast or human counterparts, dPrx5 (Drosophila peroxiredoxin 5) is expressed in several cellular compartments, including the cytosol, nucleus and the mitochondrion. Global overexpression of dPrx5 in flies increased resistance to oxidative stress and extended their life span by up to 30% under normal conditions. The dprx5(-/-) null flies were comparatively more susceptible to oxidative stress, had higher incidence of apoptosis, and a shortened life span. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling) analysis revealed that the dprx5(-/-) null mutant had discernible tissue-specific apoptotic patterns, similar to those observed in control flies exposed to paraquat. In addition, apoptosis was particularly notable in oenocytes. During development the dPrx5 levels co-varied with ecdysone pulses, suggesting inter-relationship between ecdystreroids and dPrx5 expression. The importance of dPrx5 for development was further underscored by the embryonic lethal phenotype of progeny derived from the dprx5(-/-) null mutant. Results from the present study suggest that the antioxidant and anti-apoptotic activities of dPrx5 play a critical role in development and aging of the fly.