Flurbiprofen in rapid eye movement sleep deprivation induced hyperalgesia

• Published in: Physiology & behavior Vol. 128; pp. 155 - 158
• Main Authors: Gürel, Elif Ezgi, Ural, Keremcan, Öztürk, Gülnur, Öztürk, Levent
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 10-04-2014; Elsevier
• Subjects: Analgesics - therapeutic use; Animals; Anti-nociceptive; Behavioral psychophysiology; Biological and medical sciences; Dose-Response Relationship, Drug; Female; Flurbiprofen; Flurbiprofen - therapeutic use; Fundamental and applied biological sciences. Psychology; Hot Temperature; Hyperalgesia - etiology; Hyperalgesia - physiopathology; Pain threshold; Pain Threshold - drug effects; Psychiatry; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rats; Rats, Sprague-Dawley; REM deprivation; Sleep Deprivation - complications; Sleep Deprivation - physiopathology; Sleep loss; Anti-nociceptive; Sleep loss; REM deprivation; Pain threshold; Flurbiprofen; Nociception; Prostaglandin-endoperoxide synthase; Deprivation; Enzyme; Enzyme inhibitor; Rapid eye movement sleep; Hyperalgesia; Non steroidal antiinflammatory agent; Pain; Arylpropionic acid derivatives; Sleep wake cycle; Oxidoreductases
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2014.02.003

Abstract Background Rapid eye movement (REM) sleep deprivation induces hyperalgesia in healthy rats. Here, we evaluated the effects of flurbiprofen, an anti-inflammatory and neuroprotective agent, on the increased thermal responses observed in REM sleep deprived rats. Methods Forty female rats were divided into four groups following 96-hour REM sleep deprivation: intraperitoneal injections of placebo, and flurbiprofen 5 mg/kg, 15 mg/kg and 40 mg/kg were made in CONT (n = 10), FBP5, FBP15 and FBP40 groups respectively. Pain threshold measurements were performed three times at baseline (0.hour), at the end of REM sleep deprivation (96.hour) and at 1 h after injections (97.hour) by hot plate and tail-flick tests. Results REM sleep deprivation induced a significant decrease in pain thresholds of all rats (hotplate: 0.hour vs 96.hour, 9.75 ± 2.85 vs 5.10 ± 2.02, p < 0.001; tail flick: 0.hour vs 96.hour, 11.92 ± 4.62 vs 7.92 ± 5.15, p < 0.001). Flurbiprofen in 15 mg/kg and 40 mg/kg doses significantly improved pain tolerance measured by tail flick test (tail flick in FBP15 and FBP40 groups: 96.hour vs 97.hour, 7.01 ± 4.97 vs 8.34 ± 3.61 and 5.06 ± 1.57 vs 7.04 ± 2.49, p < 0.05 for both). Conclusion 96 h of REM sleep deprivation resulted in reduced pain thresholds in both hot plate and tail flick tests. Flurbiprofen was used for the first time in a rat model of REM sleep deprivation, and it provided anti-nociceptive effects in 15 mg/kg and 40 mg/kg doses. Flurbiprofen may have the potential for treatment of painful syndromes accompanying insomnia or sleep loss.