Endothelial cell migration on surfaces modified with immobilized adhesive peptides

• Published in: Biomaterials Vol. 21; no. 17; pp. 1725 - 1733
• Main Authors: Kouvroukoglou, Stylianos, Dee, Kay C, Bizios, Rena, McIntire, Larry V, Zygourakis, Kyriacos
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-09-2000; Elsevier Science
• Subjects: Adhesion; Adhesive peptides; Amino Acid Sequence; Amino acids; Animals; Biological and medical sciences; Cattle; Cell Adhesion; Cell Movement; Cell physiology; Cells; Cells, Cultured; Chlorine compounds; Endothelial cell migration; Endothelium, Vascular - cytology; Fundamental and applied biological sciences. Psychology; Grafts; Molecular and cellular biology; Motility and taxis; Peptides - chemistry; Persistent random walk; Space life sciences; Surface modification; Surface treatment; Synthetic vascular grafts; Synthetic vascular grafts; Endothelial cell migration; Surface modification; Adhesive peptides; Persistent random walk; Implanted material; Endothelial cell; Random walk model; Spatial distribution; Peptides; Fluorescent labelling; Migration; Surface properties; Immobilized cell; Adhesion; Biomedical engineering; Non-programmatic
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(99)00205-7

Endothelial cell (EC) migration has been studied on aminophase surfaces with covalently bound RGDS and YIGSRG cell adhesion peptides. The fluorescent marker dansyl chloride was used to quantify the spatial distribution of the peptides on the modified surfaces. Peptides appeared to be distributed in uniformly dispersed large clusters separated by areas of lower peptide concentrations. We employed digital time-lapse video microscopy and image analysis to monitor EC migration on the modified surfaces and to reconstruct the cell trajectories. The persistent random walk model was then applied to analyze the cell displacement data and compute the mean root square speed, the persistence time, and the random motility coefficient of EC. We also calculated the time-averaged speed of cell locomotion. No differences in the speed of cell locomotion on the various substrates were noted. Immobilization of the cell adhesion peptides (RGDS and YIGSRG), however, significantly increased the persistence of cell movement and, thus, the random motility coefficient. These results suggest that immobilization of cell adhesion peptides on the surface of implantable biomaterials may lead to enhanced endothelization rates.