Changes in nuclear inositol phospholipids induced in intact cells by insulin-like growth factor I

• Published in: Biochemical and biophysical research communications Vol. 159; no. 2; p. 720
• Main Authors: Cocco, L, Martelli, A M, Gilmour, R S, Ognibene, A, Manzoli, F A, Irvine, R F
• Format: Journal Article
• Language: English
• Published: United States 15-03-1989
• Subjects: Animals; Cell Line; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Fibroblasts - drug effects; Fibroblasts - metabolism; Inositol Phosphates - metabolism; Insulin-Like Growth Factor I - pharmacology; Mice; Phosphatidylinositols - metabolism; Somatomedins - pharmacology
• ISSN: 0006-291X
• DOI: 10.1016/0006-291X(89)90054-5

Swiss 3T3 cells were labelled for 36 hours with high levels of 3H-myo-inositol and the radioactivity in nuclear inositol phospholipids was measured. Treatment of cells for 2 minutes, but not for 4 hours, with mitogenic concentrations of insulin-like growth factor I and bombesin caused a slight decrease in PtdIns and more marked decreases in PtdInsP and PtdInsP2. These effects were not seen if isolated nuclei were incubated with IGF-I and bombesin. We interpret these results to mean that rapid mass changes occur in nuclear inositol phospholipids in the early stages of the mitotic response.