The effect of manganese nanoparticles on performance, redox reactions and epigenetic changes in turkey tissues

• Published in: Animal (Cambridge, England) Vol. 13; no. 6; pp. 1137 - 1144
• Main Authors: Ognik, K., Kozłowski, K., Stępniowska, A., Szlązak, R., Tutaj, K., Zduńczyk, Z., Jankowski, J.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01-06-2019; Elsevier Limited; Elsevier
• Subjects: 8-Hydroxydeoxyguanosine; Antioxidants; Ascorbic acid; Blood; Blood levels; Carbonyls; Catalase; Ceruloplasmin; Deoxyribonucleic acid; Design factors; Diet; DNA; DNA damage; DNA methylation; Dosage; Enzymes; Epigenetics; Ethics; Excretion; Gene expression; Glutathione; Glutathione peroxidase; Lipid peroxidation; Lipids; Macromolecules; Malondialdehyde; Manganese; Manganese oxides; nanomanganese; Nanomaterials; Nanoparticles; Nitration; Nitrotyrosine; Nutrition; Nutrition research; Oxidation; Oxidative stress; Peroxidase; Poultry; Proteins; rearing; Redox reactions; Research Article; Studies; Superoxide dismutase; Vitamin C; Wildfowl; Poland; Germany; nanomanganese; oxidation; DNA methylation; poultry; rearing
• ISSN: 1751-7311; 1751-732X
• DOI: 10.1017/S1751731118002653

The hypothesis of the research was the assumption, that manganese nanoparticles can affect the body in the same way as macromolecules. Their smaller size and greater biological reactivity will potentially allow the Mn addition to the diet to be reduced and, consequently, less excretion of this element into the environment. The aim of the study was to determine whether the use of Mn nanoparticles would make it possible to reduce the level of this micronutrient added to turkey diets without adversely affecting redox reactions in cells and epigenetic changes. The experiment was conducted on six groups with 10 replications, in a two-factor design with three dosages of manganese: 100, 50 and 10 mg/kg, and two sources: manganese oxide (MnO) and manganese nanoparticles (NP-Mn2O3). Markers of oxidative stress determined in the blood, that is, the concentration of lipid hydroperoxides, malondialdehyde, protein carbonyl derivatives, 3-nitrotyrosine, 8-hydroxydeoxyguanosine, total glutathione, superoxide dismutase, glutathione peroxidase, catalase, ceruloplasmin, total antioxidant status, albumin and vitamin C content. The level of epigenetic changes in the blood was determined by analysing global DNA methylation. In the experiment, in which the diet of turkeys was supplemented with two forms of Mn (MnO or NP-Mn2O3) at three dosages: 100, 50 and 10 mg/kg, the 10 mg/kg dose, especially in the form of NP-Mn2O3, induced lipid oxidation reactions to the greatest extent. Irrespective of the dosage of Mn in the turkey diet, Mn in the form of NP-Mn2O3 was found to reduce protein nitration more than Mn in the form of MnO. Reducing the Mn dosage in the diet from 100 to 50 mg/kg and then to 10 mg/kg is unfavourable because proportionally increases protein and DNA oxidation in cells, decreases the activity of antioxidant enzymes, and increases the level of glutathione. Reducing the dosage from 100 to 10 mg/kg increases global DNA methylation. The reduction of the Mn level, regardless of the form used, is disadvantageous, because it weakens the defense of the antioxidant system, which consequently can induce oxidative processes in the cells. Although Mn in the form of NP-Mn2O3 reduce protein nitration better than in MnO form, the use of manganese nanoparticles in turkey feeding (even in lower doses) requires further study.