Antiproliferative, Antioxidant, Chemopreventive and Antiangiogenic Potential of Chromatographic Fractions from Anemonia sulcata with and without Its Symbiont Symbiodinium in Colorectal Cancer Therapy

• Published in: International journal of molecular sciences Vol. 24; no. 14; p. 11249
• Main Authors: Peña, Mercedes, Mesas, Cristina, Perazzoli, Gloria, Martínez, Rosario, Porres, Jesús M, Doello, Kevin, Prados, Jose, Melguizo, Consolación, Cabeza, Laura
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08-07-2023; MDPI
• Subjects: Alzheimer's disease; Anemonia sulcata; antiangiogenic activity; antioxidant activity; Antioxidants; Biodiversity; Cancer therapies; chemopreventive activity; Chromatography; Clinical trials; Colorectal cancer; Cytotoxicity; Drugs; fractionation; Medical prognosis; Protein expression; Proteins; Quality of life; Symbiodinium; fractionation; colorectal cancer; chemopreventive activity; antitumor activity; Anemonia sulcata; antiangiogenic activity; antioxidant activity; Symbiodinium
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241411249

may be a source of marine natural products (MNPs) due to the antioxidant and antitumor activity of its crude homogenates shown in vitro in colon cancer cells. A bioguided chromatographic fractionation assay of crude
homogenates with and without its symbiont
was performed to characterize their bioactive composition and further determine their biological potential for the management of colorectal cancer (CRC). The 20% fractions retained the in vitro antioxidant activity previously reported for homogenates. As such, activation of antioxidant and detoxifying enzymes was also evaluated. The 40% fractions showed the greatest antiproliferative activity in T84 cells, synergistic effects with 5-fluoruracil and oxaliplatin, overexpression of apoptosis-related proteins, cytotoxicity on tumorspheres, and antiangiogenic activity. The predominantly polar lipids and toxins tentatively identified in the 20% and 40% fractions could be related to their biological activity in colon cancer cells although further characterizations of the active fractions are necessary to isolate and purify the bioactive compounds.