Molecular and Physiological Evaluation of Bread Wheat ( Triticum aestivum L.) Genotypes for Stay Green under Drought Stress

• Published in: Genes Vol. 13; no. 12; p. 2261
• Main Authors: Zada, Ahmad, Ali, Ahmad, Binjawhar, Dalal Nasser, Abdel-Hameed, Usama K, Shah, Azhar Hussain, Gill, Shahid Maqsood, Hussain, Irtiza, Abbas, Zaigham, Ullah, Zahid, Sher, Hassan, Ali, Iftikhar
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30-11-2022; MDPI
• Subjects: Agricultural production; Analysis; Bread; Carbon dioxide; China; Chlorophyll; Cultivars; Dehydration; Drought; Drought resistance; Droughts; Gene expression; Gene families; Genes; Genetic aspects; Genotype; Genotypes; Hardiness; Harvest; Influence; Light-harvesting complex; Molecular modelling; Photosynthesis; Photosystem II; Physiological aspects; Physiology; Plants; Proteins; Stress (Physiology); Triticum; Triticum aestivum; Water availability; Water content; Water shortages; Water stress; Wheat; China; United States > US; LHCI; drought stress; stay green; gene expression; LHCII
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes13122261

Water availability is considered as the main limiting factor of wheat growth illuminating the need of cultivars best adapted to drought situations for better wheat production and yield. Among these, the stay-green trait is thought to be related to the ability of wheat plants to maintain photosynthesis and CO
assimilation, and a detailed molecular understanding of this trait may help in the selection of high-yielding, drought-tolerant wheats. The current study, therefore, evaluated the physiological responses of the selected wheat genotypes under pot-induced water stress conditions through different field capacities. The study also focused on exploring the molecular mechanisms involved in drought tolerance conferred due to the stay-green trait by studying the expression pattern of the selected PSI-associated light-harvesting complex I (LHC1) and PSII-associated LHCII gene families related to pigment-binding proteins. The results revealed that the studied traits, including relative water content, membrane stability index and chlorophyll, were variably and negatively affected, while the proline content was positively enhanced in the studied wheats under water stress treatments. Molecular diagnosis of the selected wheat genotypes using the expression profile of 06 genes, viz.
,
,
,
,
and
that encodes for the LHCI and LHCII proteins, indicated variable responses to different levels of drought stress. The results obtained showed the relation between the genotypes and the severity of the drought stress condition. Among the studied genotypes, Chirya-1 and SD-28 performed well with a higher level of gene expression under drought stress conditions and may be used in genetic crosses to enrich the genetic background of common wheat against drought stress.