Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma

• Published in: Acta pharmaceutica (Zagreb, Croatia) Vol. 71; no. 4; pp. 587 - 602
• Main Authors: Karakurt, Serdar, Kandir, Sinan, Gökçek-Saraç, Çiğdem
• Format: Journal Article
• Language: English
• Published: Poland Sciendo 01-12-2021; De Gruyter Poland
• Subjects: Acids; Antioxidants; Apoptosis; cell viability; Colorectal cancer; Colorectal carcinoma; Cytoplasm; In vivo methods and tests; Invasiveness; Metastases; Molecular modelling; NQO1; p53; p53 Protein; Proteins; Tannic acid; Up-regulation; wound healing; NQO1; wound healing; tannic acid; cell viability; colorectal carcinoma; p53
• ISSN: 1846-9558; 1330-0075; 1846-9558
• DOI: 10.2478/acph-2021-0036

The present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620 and HT-29 with
values of 7.2 ± 0.8 and 37.6 ± 1.4 µmol L
. Besides, metastatic, invasive, and colony formation properties of CRC cells were significantly inhibited following the tannic acid treatment (
< 0.001). Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (
< 0.001), which leads the cells to trigger apoptosis. In conclusion, the present
study may supply a significant background for
studies in which the molecular mechanisms of antioxidant and chemopreventive activities of tannic acid will completely clarify.