Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence Lycoris sprengeri Bulblet Regeneration In Vitro

• Published in: International journal of molecular sciences Vol. 22; no. 21; p. 11890
• Main Authors: Ren, Ziming, Xu, Yunchen, Lvy, Xuesi, Zhang, Dong, Gao, Cong, Lin, Yefan, Liu, Yue, Wu, Yun, Xia, Yiping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02-11-2021; MDPI
• Subjects: 6-benzyladenine; Benzyladenine; beta-Fructofuranosidase - genetics; beta-Fructofuranosidase - metabolism; Biological activity; bulblet regeneration; Carbohydrates; Cell division; Cell Wall - enzymology; cell wall invertase; Cell walls; Cleavage; Degradation; Efficiency; endogenous hormone; Explants; Flowering; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Hormones; Invertase; Lycoris; Lycoris - genetics; Lycoris - growth & development; Lycoris - metabolism; Lycoris sprengeri; Metabolism; Nutrients; Plant growth; Plant Stems - genetics; Plant Stems - growth & development; Plant Stems - metabolism; Sucrose; Sucrose - metabolism; sucrose cleavage; Sucrose synthase; Trends; endogenous hormone; 6-benzyladenine; cell wall invertase; Lycoris sprengeri; sucrose cleavage; bulblet regeneration
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222111890

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For
species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of
(
) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of
than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.